Morphogen-defined patterning of Escherichia coli enabled by an externally tunable band-pass filter.
Morphogen-defined patterning of Escherichia coli enabled by an externally tunable band-pass filter.
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DOI：
10.1186/1754-1611-3-10
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发表时间：
2009-07-08
影响因子：
5.6
通讯作者：
Ostermeier M
中科院分区：
文献类型：
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作者：
Sohka T;Heins RA;Ostermeier M
形态发生因子决定细胞的命运-这是一种在发育过程中特别重要的现象。一个简单的模型系统，研究形态发生素如何模式细胞行为将克服固有的困难，在体内研究天然形态发生素。建立这样一个系统的合成生物学方法是有吸引力的。使用外部可调带通滤波器范例，我们设计了大肠杆菌细胞作为模型系统，用于研究多种形态发生素如何影响细胞行为。我们展示了我们的系统如何表现出行为，如形态发生剂串扰，以及细胞的生长和荧光如何以许多复杂的模式进行图案化。我们将我们的细胞模式从平板表面的2D培养物扩展到软琼脂培养基中的3D培养物。我们的系统提供了一个方便的，定义明确的模型系统的基础研究如何多形态梯度可以影响细胞的命运，并导致模式的形成。我们的设计原理可以应用于真核细胞，以开发其他模型系统，用于研究发育或使细胞图案化，用于组织工程和生物材料等应用。
Gradients of morphogens pattern cell fate – a phenomenon that is especially important during development. A simple model system for studying how morphogens pattern cell behavior would overcome difficulties inherent in the study of natural morphogens in vivo. A synthetic biology approach to building such a system is attractive. Using an externally-tunable band-pass filter paradigm, we engineered Escherichia coli cells to function as a model system for the study of how multiple morphogens can pattern cell behavior. We demonstrate how our system exhibits behavior such as morphogen crosstalk and how the cells' growth and fluorescence can be patterned in a number of complex patterns. We extend our cell patterning from 2D cultures on the surface of plates to 3D cultures in soft agarose medium. Our system offers a convenient, well-defined model system for fundamental studies on how multiple morphogen gradients can affect cell fate and lead to pattern formation. Our design principles could be applied to eukaryotic cells to develop other models systems for studying development or for enabling the patterning of cells for applications such as tissue engineering and biomaterials.