Characterization of putative soluble and membrane-bound trehalases in a hemipteran insect, Nilaparvata lugens
Characterization of putative soluble and membrane-bound trehalases in a hemipteran insect, Nilaparvata lugens
复制标题
DOI：
10.1016/j.jinsphys.2009.07.003
复制
发表时间：
2009-11-01
影响因子：
2.2
通讯作者：
Zhang, Yongjun
中科院分区：
文献类型：
--
作者：
Gu, Jianhua;Shao, Ying;Zhang, Yongjun
海藻糖是昆虫的主要血糖，海藻糖酶参与细胞内能量代谢和控制海藻糖水平。从褐飞虱体内鉴定出两种形式(可溶性和膜结合型)的海藻糖酶及其相应的基因(Nitre-1和Nitre-2)。Nitre-1和Nitre-2都含有海藻糖酶特征基序，Nitre-2含有一个可能的跨膜结构域。比较不同发育阶段和20-羟基蜕皮酮(20E)处理后的海藻糖酶活性和基因mRNA水平，表明Nitre-1和Nitre-2分别编码一种可溶性海藻糖酶和一种膜结合海藻糖酶。褐飞虱可溶性海藻糖酶活性占总海藻糖酶6活性的大部分。20E只能诱导可溶性海藻糖酶活性和Nitre-1mRNA水平。此外，长翅型个体只有可溶性海藻糖酶活性显著高于短翅型个体。这些结果表明，只有可溶性海藻糖酶在长翅目和短翅目昆虫中有不同的表达，并且对激素刺激更敏感。(C)2009爱思唯尔有限公司。保留所有权利。
Trehalose is the main blood sugar of insects, and the enzyme trehalase is involved in energy metabolism and controlling trehalose levels in cells. Two forms (soluble and membrane-bound) of trehalase and the corresponding genes (NITre-1 and NITre-2) were identified from the brown planthopper, Nilaparvata lugens. Both NITre-1 and NITre-2 contain trehalase signature motifs, and NITre-2 contains a putative transmembrane domain. Comparison of trehalase activity and gene mRNA level at different developmental stages, or following application of 20-hydroxyecdysone (20E), suggests that NITre-1 and NITre-2 encode a soluble trehalase and a membrane-bound trehalase respectively. Soluble trehalase activity accounted for the majority of total trehalas6 activity in N. lugens. Only soluble trehalase activity and NITre-1 mRNA level could be induced by 20E. Additionally, only soluble trehalase activity was significantly higher in macropterous individuals than in brachypterous morphs. These results indicate that only soluble trehalase is differentially expressed between macropterous and brachypterous individuals and is more responsive to hormone stimulus. (C) 2009 Elsevier Ltd. All rights reserved.