Freeze-gelled alginate/gelatin scaffolds for wound healing applications: An in vitro, in vivo study
Freeze-gelled alginate/gelatin scaffolds for wound healing applications: An in vitro, in vivo study
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DOI：
10.1016/j.msec.2020.110957
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发表时间：
2020-08-01
影响因子：
7.9
通讯作者：
Kamali, Ali
中科院分区：
文献类型：
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作者：
Afjoul, Homa;Shamloo, Amir;Kamali, Ali
本研究采用冷冻凝胶法制备了三维多孔支架。最近，由于其独特的可调谐特性，使用聚合物共混物制造支架已在许多组织工程应用中变得普遍。在这项工作中，我们在冷冻-凝胶法的基础上用一种新的方法制备了用于伤口愈合的海藻酸盐-明胶多孔水凝胶。将海藻酸盐和明胶以三种不同的比例混合，得到的溶液经过冷冻凝胶化得到3D多孔基质。我们使用不同的表征测试对样品进行了分析。扫描电子显微镜(SEM)结果表明，冷冻凝胶法成功地获得了所有海藻酸盐-明胶样品的多孔形貌，这与以前使用该方法制备单聚合物时所看到的情况一样。藻胶比影响基质的溶胀、生物降解性、细胞培养和力学性能。明胶含量最低的支架具有最高的膨胀率，随着明胶含量的增加，生物降解率、细胞增殖和活力增加。在力学性能方面，随着明胶含量的增加，支架的延展性更强，拉伸强度也更高。体内实验结果还表明，该复合支架具有良好的生物相容性，对大鼠创面愈合过程具有积极作用。本研究所采用的制备海藻酸盐-明胶多孔支架的低成本方法可以进行调整和修改，以适应不同的组织工程应用。
In this study, fabrication of a three-dimensional porous scaffold was performed using freeze gelation method. Recently, fabrication of scaffolds using polymer blends has become common for many tissue engineering applications due to their unique tunable properties. In this work, we fabricated alginate-gelatin porous hydrogels for wound healing application using a new method based on some modifications to the freeze-gelation method. Alginate and gelatin were mixed in three different ratios and the resulting solutions underwent freeze gelation to obtain 3D porous matrices. We analyzed the samples using different characterization tests. The scanning electron microscopy (SEM) results indicated that the freeze gelation method was successful in obtaining porous morphologies for all the fabricated alginate-gelatin samples as previously was seen in single-polymer fabrication using this method. The alginate to gelatin ratio affected swelling, biodegradation, cell culture and mechanical properties of the matrices. The scaffold with the lowest content of gelatin had the highest swelling ratio while biodegradation and cell proliferation and viability were increased with the gelatin content. Regarding the mechanical properties, as the gelatin content increased, the scaffold became more ductile and showed higher tensile strength. The in-vivo results also showed the biocompatibility of the blend scaffold and its positive role in wound healing process in rats. The low-cost procedure used in this study to fabricate the porous alginate-gelatin scaffolds can be adapted and modified to suit different tissue engineering applications.