Expression localisation and functional activity of pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide and their receptors in mouse ovary
Expression localisation and functional activity of pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide and their receptors in mouse ovary
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DOI：
10.1530/rep-07-0051
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发表时间：
2007-08-01
期刊：
影响因子：
3.8
通讯作者：
Canipari, Rita
中科院分区：
文献类型：
--
作者：
Barberi, Marzia;Muciaccia, Barbara;Canipari, Rita
腺苷酸环化酶激活多肽（PACAP）和血管活性肠肽（VIP）对与排卵过程相关的几个参数有积极影响。PACAP在大鼠排卵前卵泡中瞬时表达，而VIP在发育的所有阶段都存在于神经纤维中。这两种肽通过与三种类型的受体相互作用而发挥作用：PACAP I型受体（PAC 1-R），其与PACAP的亲和力更高，以及两种VIP受体（VPAC 1-R和VPAC 2-R），其与PACAP和VIP以相等的亲和力结合。本研究的目的是研究小鼠卵巢中PACAPNIP/受体系统。RT-PCR、免疫组化和原位杂交结果表明，在人绒毛膜促性腺激素（hCG）刺激后，排卵前卵泡颗粒细胞中PACAP有瞬时表达，而VIP mRNA未见表达。所有受体均存在于22日龄的未处理小鼠中。在排卵前卵泡中，PAC 1-R在颗粒细胞和残留的卵巢组织中表达，但主要在颗粒细胞中受到hCG的刺激; VPAC 2-R存在于两个细胞室中，并且仅受到轻度刺激; VPAC 1-R主要存在于残留的卵巢组织中，并且被hCG下调。PACAP和VIP在抑制颗粒细胞凋亡方面是等效的，证实了功能性PACAPNIP受体的存在。hCG对排卵前卵泡颗粒细胞中PACAP和PAC 1-R的当代诱导表明，在小鼠卵巢中，PACAP也可能在排卵前后发挥重要作用。此外，PACAPNIP受体在未处理的卵巢中的存在表明PACAP和VIP在卵泡发育过程中可能起作用。
Pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) positively affect several parameters correlated with the ovulatory process. PACAP is transiently expressed in rat preovulatory follicles, while VIP is present in nerve fibres at all stages of development. These two peptides act by interacting with three types of receptors: PACAP type I receptor (PAC1 -R), which binds with higher affinity to PACAP, and two VIP receptors (VPAC1 -R and VPAC2-R), which bind to PACAP and VIP with equal affinity. The aim of the present study was to characterise the PACAPNIP/receptor system in the mouse ovary. Results obtained by RT-PCR, immunohistochemistry and in situ hybridisation showed that PACAP was transiently expressed in granulosa cells of preovulatory follicles after human chorionic gonadotrophin (hCG) stimulation, while VIP mRNA was never observed. All the receptors were present in 22-day-old untreated mice. In preovulatory follicles, PAC1-R was expressed both in granulosa cells and in residual ovarian tissue but was stimulated by hCG mainly in granulosa cells; VPAC2-R was present in both the cell compartments and was only mildly stimulated; VPAC1 -R was present mainly in the residual ovarian tissue and was downregulated by hCG. PACAP and VIP were equipotent in inhibiting apoptosis in granulosa cells, confirming the presence of functional PACAPNIP receptors. The contemporary induction by hCG of PACAP and PAC1 -R in granulosa cells of preovulatory follicles suggests that, also in mouse ovary, PACAP may play a significant role around the time of ovulation. Moreover, the presence of PACAPNIP receptors in the untreated ovary suggests a possible role for PACAP and VIP during follicle development.