A link between RNA interference and nonsense-mediated decay in Caenorhabditis elegans
A link between RNA interference and nonsense-mediated decay in Caenorhabditis elegans
复制标题
DOI：
10.1126/science.289.5486.1928
复制
发表时间：
2000-09-15
期刊：
影响因子：
56.9
通讯作者：
Mango, SE
中科院分区：
文献类型：
--
作者：
Domeier, ME;Morse, DP;Mango, SE
双链RNA（dsRNA）通过信使RNA降解抑制同源基因的表达。为了深入了解降解机制，我们研究了RNA干扰如何受到smg基因突变的影响，这是无义介导的衰变所必需的。对于测试的六个smg基因中的三个，突变导致动物最初被dsRNA沉默，但随后恢复;野生型动物保持沉默。在恢复期间，靶信使RNA的水平恢复，并且dsRNA的RNA编辑和降解与野生型的那些相同。我们认为RNA干扰的持久性依赖于smg基因的一个子集。
Double-stranded RNA (dsRNA) inhibits expression of homologous genes by a process' involving messenger RNA degradation. To gain insight into the mechanism of degradation, we examined how RNA interference is affected by mutations in the smg genes, which are required for nonsense-mediated decay. For three of six smg genes tested, mutations resulted in animals that were initially silenced by dsRNA but then recovered; wild-type animals remained silenced. The Levels of target messenger RNAs were restored during recovery, and RNA editing and degradation of the dsRNA were identical to those of the wild type. We suggest that persistence of RNA interference relies on a subset of smg genes.