In Vivo Outcomes of Tissue-Engineered Osteochondral Grafts
In Vivo Outcomes of Tissue-Engineered Osteochondral Grafts
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DOI：
10.1002/jbm.b.31571
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发表时间：
2010-04-01
影响因子：
3.4
通讯作者：
Cook, James L.
中科院分区：
文献类型：
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作者：
Bal, B. Sonny;Rahaman, Mohamed N.;Cook, James L.
组织工程骨软骨移植物已由多种材料合成，在修复动物模型中的软骨缺陷方面取得了一些成功。我们假设，在通过将负载间充质干细胞的水凝胶粘合到多孔材料上合成的组织工程骨软骨移植物中，多孔支架的选择将影响移植物对宿主骨的愈合以及透明软骨表面细胞修复的质量。骨髓来源的同种异体间充质干细胞悬浮在水凝胶中，水凝胶附着在多孔钽金属、同种异体移植骨或生物活性玻璃的圆柱体上。由此产生的组织工程骨软骨移植物被植入兔子膝盖的实验性缺损处。在 6 周和 12 周时比较了三种软骨下材料的软骨下骨修复、缺损填充、骨长入-种植体整合和关节组织质量。生物活性玻璃和多孔钽在与邻近宿主骨的整合、移植物表面再生透明样组织以及在关节软骨中表达 II 型胶原方面优于同种异体骨移植物。 (C) 2010 Wiley periodicals, Inc. J Biorned Mater Res Pan B：Appl Biotnater 93B：164-174，2010
Tissue-engineered osteochondral grafts have been synthesized from a variety of materials, with some success at repairing chondral defects in animal models. We hypothesized that in tissue-engineered osteochondral grafts synthesized by bonding mesenchymal stem cell-loaded hydrogels to a porous material, the choice of the porous scaffold would affect graft healing to host bone, and the quality of cell restoration at the hyaline cartilage surface. Bone marrow-derived allogeneic mesenchymal stem cells were suspended in hydrogels that were attached to cylinders of porous tantalum metal, allograft bone, or a bioactive glass. The tissue-engineered osteochondral grafts, thus created were implanted into experimental defects in rabbit knees. Subchondral bone restoration, defect fill, bone ingrowth-implant integration, and articular tissue quality were compared between the three subchondral materials at 6 and 12 weeks. Bioactive glass and porous tantalum were superior to bone allograft in integrating to adjacent host bone, regenerating hyaline-like tissue at the graft surface, and expressing type II collagen in the articular cartilage. (C) 2010 Wiley Periodicals, Inc. J Biorned Mater Res Pan B: Appl Biotnater 93B: 164-174, 2010