Structure of the replicating complex of a pol α family DNA polymerase
Structure of the replicating complex of a pol α family DNA polymerase
复制标题
DOI：
10.1016/s0092-8674(01)00367-1
复制
发表时间：
2001-06-01
期刊：
影响因子：
64.5
通讯作者：
Steitz, TA
中科院分区：
文献类型：
--
作者：
Franklin, MC;Wang, JM;Steitz, TA
我们描述了带有引物 - 板DNA和DTTP的RB69 DNA聚合酶的2.6 Angstrom分辨率晶体结构，在引物延伸前捕获了该步骤。人DNA聚合酶α家族中的这种三元复合物结构相对于猿猴蛋白结构显示了60度的旋转，类似于pol I家族聚合酶中的手指运动。底漆3'末端附近的小凹槽相互作用提出了Pol I和pol Alpha家族聚合酶的常见保真度机制。双链产物DNA取向在聚合模式和编辑模式结构之间差异40度。拇指在这种DNA运动中的作用为Pol Alpha家族中的编辑提供了模型。
We describe the 2.6 Angstrom resolution crystal structure of RB69 DNA polymerase with primer-template DNA and dTTP, capturing the step just before primer extension. This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation of the fingers domain relative to the ape-protein structure, similar to the fingers movement in pol I family polymerases. Minor groove interactions near the primer 3' terminus suggest a common fidelity mechanism for pol I and pol alpha family polymerases. The duplex product DNA orientation differs by 40 degrees between the polymerizing mode and editing mode structures. The role of the thumb in this DNA motion provides a model for editing in the pol alpha family.