Expression of estrogen receptor-α, estrogen receptor-β and placental endothelial and inducible NO synthase in intrauterine growth-restricted and normal placentas
Expression of estrogen receptor-α, estrogen receptor-β and placental endothelial and inducible NO synthase in intrauterine growth-restricted and normal placentas
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DOI：
10.1016/j.arcmed.2006.03.011
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发表时间：
2006-11-01
影响因子：
7.7
通讯作者：
Jeschke, Udo
中科院分区：
文献类型：
--
作者：
Schiessl, Barbara;Mylonas, Ioannis;Jeschke, Udo
背景一氧化氮似乎在胎盘血液循环的生理学中起重要作用，尽管其在病理性胎盘中的表达及其作用仍不清楚。因此，本研究的目的是调查雌激素受体α（ER α），雌激素受体β（ER β）和内皮型一氧化氮合酶（eNOS）和诱导型一氧化氮合酶（iNOS）在宫内生长受限（IUGR）胎盘，溶血，肝酶升高，血小板减少（HELLP）胎盘和正常健康对照胎盘中的表达。从诊断为IUGR、HELLP和正常足月胎盘的患者分娩后获得石蜡包埋的胎盘组织切片，并分析eNOS和iNOS以及ER α/β表达。采用半定量评分法分析免疫组化反应强度，并进行统计学分析。此外，还进行了Western blot实验，以比较免疫组化和Western blot获得的染色强度。HELLP患者胎盘滋养层细胞eNOS和iNOS的表达明显降低。然而，与正常对照相比，HELLP胎盘中的ER表达表现出显著升高的表达强度。ER α的表达在所有三种研究的病理中没有显著差异。我们推测，合体滋养层和绒毛外滋养层细胞中ER β表达的增加代表胎盘组织的加速增殖，或者可以被视为HELLP胎盘的补偿效应。(C)2006年IMSS。爱思唯尔公司出版
Background. Nitric oxide seems to play important roles in the physiology of placental blood circulation, although their expression in pathological placentas and their role is still unclear. Therefore, the aim of this study was to investigate the expression of estrogen receptor alpha (ER alpha), estrogen receptor beta (ER beta) and the endothelial NO synthase (eNOS) and inducible NO synthase (iNOS) in intrauterine growth-restricted (IUGR) placentas, hemolysis, elevated liver enzymes, low platelets (HELLP) placentas and in normal healthy control placentas.Methods. Slides of paraffin-embedded placental tissue were obtained after delivery from patients diagnosed with IUGR, HELLP and normal term placentas and analyzed for eNOS and iNOS, as well as ER alpha/beta expression. Intensity of immunohistochemical reaction was analyzed using a semi-quantitative score, and statistical analysis was performed. In addition, Western blot experiments were performed for comparison of staining intensities obtained by immunohistochemistry and Western blot.Results. Expression of eNOS and iNOS is significantly reduced in trophoblast cells of placentas with HELLP. However, ER expression in HELLP placentas demonstrated a significantly elevated expression intensity compared to normal controls. ERa expression was not significantly different in all three pathologies investigated.Conclusions. We speculate that the increased ER beta expression in both syncytiotrophoblast and extravillous trophoblast cells represents accelerated proliferation of placental tissue or can be seen as a compensatory effect in HELLP placentas. (C) 2006 IMSS. Published by Elsevier Inc.