THE EFFECT OF SHEAR RATE ON PLATELET INTERACTION WITH SUBENDOTHELIUM EXPOSED TO CITRATED HUMAN-BLOOD
THE EFFECT OF SHEAR RATE ON PLATELET INTERACTION WITH SUBENDOTHELIUM EXPOSED TO CITRATED HUMAN-BLOOD
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DOI：
10.1016/0026-2862(80)90054-0
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发表时间：
1980-01-01
影响因子：
3.1
通讯作者：
BAUMGARTNER, HR
中科院分区：
文献类型：
--
作者：
TURITTO, VT;WEISS, HJ;BAUMGARTNER, HR
将抗凝血至19.7 mM柠檬酸钠血浆浓度的人血暴露于环形灌注室的内皮下层，其壁剪切速率为50-10,000/s，暴露时间为0.5-40分钟。血小板粘附随着暴露时间的增加而增加，直到达到完全饱和（100%表面覆盖率）。在所有血流条件下，血小板血栓都是短暂形成的，在5-10 min时达到最大值，在40 min时最终消失。黏附速率随剪力速率的增加而增加，达到650/s；在较高的剪切值下，没有观察到明显的增加。在剪切速率为350/s以下，血小板血栓几乎不存在，但随着剪切速率增加到10000 /s，血小板血栓不断增加。血液，抗凝至血浆浓度为45 mM柠檬酸钠，剪切速率高达10000 /s，不形成血小板血栓；血小板粘附相对独立于650/s的剪切速率，在650/s以上随剪切速率的增大而减小。用经典质量传递理论分析黏附实验结果表明，随着壁面剪切速率的增大，血小板扩散系数增大，血小板-表面反应速率系数减小。这种组合导致扩散控制的反应速率低于350/s，并且相对快速地过渡到反应控制速率高于800/s的壁面剪切速率。
Human blood, anticoagulated to a plasma concentration of 19.7 mM sodium citrate, was exposed to subendothelium in an annular perfusion chamber for wall shear rates ranging from 50-10,000/s and exposure times of 0.5-40 min. Platelet adhesion increased with exposure time until complete saturation (100% surface coverage) was attained. Platelet thrombi were transiently formed under all flow conditions, reached a maximum at 5-10 min, and eventually disappeared by 40 min. Rates of adhesion increased with wall shear rate to 650/s; at higher values of shear no significant increase was observed. Platelet thrombi were virtually nonexistent below shear rates of 350/s, but increased continuously as shear rate was increased to 10,000/s. Blood, anticoagulated to a plasma concentration of 45 mM sodium citrate, formed no platelet thrombi for shear rates as high as 10,000/s; platelet adhesion was relatively independent of wall shear rate to 650/s and decreased as shear was increased above 650/s. Analysis of the experimental adhesion results with classical mass transport theory suggests that platelet diffusivity increases and platelet-surface reaction rate coefficient decreases with increasing wall shear rate. This combination results in a diffusion-controlled reaction rate below 350/s with a relatively rapid transition to a more reaction-controlled rate for wall shear rates above 800/s.