ESTABLISHMENT OF ACELLULAR EXTRINSIC FIBER CEMENTUM ON HUMAN TEETH - A LIGHT-MICROSCOPIC AND ELECTRON-MICROSCOPIC STUDY
ESTABLISHMENT OF ACELLULAR EXTRINSIC FIBER CEMENTUM ON HUMAN TEETH - A LIGHT-MICROSCOPIC AND ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1007/bf00318774
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发表时间：
1991-02-01
影响因子：
3.6
通讯作者：
SCHROEDER, HE
中科院分区：
文献类型：
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作者：
BOSSHARDT, DD;SCHROEDER, HE
本研究首次描述了早期脱细胞外源性纤维牙骨质（AEFC）在人类牙齿上形成的过程。精确选择牙根发育至终牙长度50%-100%的前磨牙，在Karnovsky固定液中固定，大多数前磨牙在EDTA中脱钙。他们的根被细分成大约10块，从近根和远根表面切割。渗透后，将这些块嵌入Epon中，并进行光镜和透射电镜切片。有些块状切割时未脱矿。在半薄染色切片上，使用Videoplan-system测量从根表面突出的胶原纤维条纹的密度。纤维条纹形成并附着于牙本质根表面后矿化，纤维条纹逐渐变长，随后矿化。条纹延伸与成矿锋面推进成正比。因此，在AEFC发育的各个阶段，短纤维条纹覆盖矿化AEFC。与AEFC厚度无关，其密度保持不变。后者逐渐增加，并在颈部区域达到早期最大值15-20 μ m。在这个阶段，AEFC边缘似乎与未来的牙龈或牙齿支撑装置的其他胶原纤维融合。边缘的矿化开始于孤立的球形或球状中心，后来与矿化锋融合并合并为AEFC。
The present study describes for the first time the development of early acellular extrinsic fiber cementum (AEFC) until its establishment on human teeth. Precisely selected premolars with roots developed to 50%-100% of their final length were prefixed in Karnovsky's fixative and most of them were decalcified in EDTA. Their roots were subdivided into about 10 blocks each, cut from the mesial and distal root surfaces. Following osmication, these blocks were embedded in Epon and sectioned for light- and transmission electron microscopy. Some blocks were cut non-demineralized. From semithin stained sections, the density of the collagenous fiber fringe protruding from the root surface was measured by using the Videoplan-system. After initiation of this fiber fringe and its attachment to the dentinal root surface followed by mineralization, the fringe gradually increased in length and subsequently became mineralized. Fringe elongation and the advancement of the mineralization front appeared to progress proportionally. Thus, in all stages of AEFC development, a short fiber fringe covered the mineralized AEFC. Its density remained constant, irrespective of AEFC thickness. The latter gradually increased and reached an early maximum of 15-20-mu-m in the cervical region. At this stage, the AEFC fringe appeared to fuse with the future dentogingival or other collagen fibers of the tooth supporting apparatus. Mineralization of the fringe commenced with isolated, spherical or globular centers, which later fused with the mineralization front and became incorporated in AEFC.