Differential regulation of mouse uncoupling proteins among brown adipose tissue, white adipose tissue, and skeletal muscle in chronic beta 3 adrenergic receptor agonist treatment.
Differential regulation of mouse uncoupling proteins among brown adipose tissue, white adipose tissue, and skeletal muscle in chronic beta 3 adrenergic receptor agonist treatment.
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慢性β3肾上腺素能受体激动剂治疗中小鼠棕色脂肪组织、白色脂肪组织和骨骼肌之间解偶联蛋白的差异调节。
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发表时间：
1998
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影响因子：
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通讯作者：
I. Tanaka
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作者：
H. Yoshitomi;K. Yamazaki;S. Abe;I. Tanaka
解偶联蛋白（UCPs）是线粒体内膜转运蛋白，其耗散质子梯度，以热的形式释放储存的能量，而不与其他能量消耗过程偶联。因此，UCP被认为是代谢效率的重要决定因素。为了阐明UCPs表达与胰岛素敏感性改善之间的关系，我们用β 3肾上腺素能受体激动剂处理KK-Ay小鼠21天，并检测各种组织中UCPs mRNA表达的变化。特异性β 3肾上腺素能受体激动剂CL 316，243的长期治疗（0.2 mg/kg体重/天s.c.）显著增加解偶联蛋白1（UCP 1）、解偶联蛋白2（UCP 2）和解偶联蛋白3（UCP 3）的表达，分别为棕色脂肪组织（BAT）的14倍、6倍和16倍。白色脂肪组织（WAT）中的UCP 1和UCP 3 mRNA表达也分别增加了12倍和9倍，但该组织中的UCP 2 mRNA表达没有变化。有趣的是，UCP 2和UCP 3 mRNA表达在骨骼肌和心脏中显著降低。特别地，骨骼肌中的UCP 3 mRNA表达水平下降到盐水处理的对照小鼠的10%，表明UCPs mRNA表达以组织特异性方式调节。血浆胰岛素和循环游离脂肪酸（FFA）浓度显著降低，表明它们与骨骼肌和心脏中UCP 2和UCP 3 mRNA表达的降低相关。UCP 1和UCP 3 mRNA在BAT和WAT中的表达主要受下丘脑通过交感神经系统的调控，而胰岛素、FFA或两者的水平可能在骨骼肌和心脏UCP 2和UCP 3 mRNA的表达调控中起重要作用。
Uncoupling proteins (UCPs) are inner mitochondrial membrane transporters that dissipate the proton gradient, releasing stored energy as heat, without coupling to other energy-consuming processes. Therefore, the UCPs are thought to be important determinants of the metabolic efficiency. To elucidate relationships between the UCPs expressions and insulin sensitivity improvement, we treated KK-Ay mice with beta 3 adrenergic receptor agonist for 21 days and examined the changes of the UCPs mRNA expressions in various tissues. Chronic treatment of a specific beta 3 adrenergic receptor agonist, CL316,243 (0.2 mg/kg body weight/day s.c.) markedly increased the expressions of uncoupling protein 1 (UCP1), uncoupling protein 2 (UCP2), and uncoupling protein 3 (UCP3) by 14-fold, 6-fold, and 16-fold, respectively, in the brown adipose tissue (BAT). The UCP1 and UCP3 mRNA expressions in the white adipose tissue (WAT) were also increased by 12-fold and 9-fold, respectively, but the UCP2 mRNA expression was not changed in this tissue. Interestingly, the UCP2 and UCP3 mRNA expressions were strikingly decreased in the skeletal muscle and heart. Particularly, the UCP3 mRNA expression level in the skeletal muscle was dropped to 10% of that of the saline-treated control mice, indicating that the UCPs mRNA expressions are regulated in tissue-specific ways. The concentrations of plasma insulin and circulating free fatty acid (FFA) were significantly decreased, suggesting that they correlate with the reductions of the UCP2 and UCP3 mRNA expressions in the skeletal muscle and heart. It has been thought that the UCP1 and UCP3 mRNA expressions in the BAT and WAT are mainly controlled by the hypothalamus via the sympathetic nervous system, while the levels of insulin, FFA or both may play important roles in the control of the UCP2 and UCP3 mRNA expressions in the skeletal muscle an heart.