PERIODIC DENSITY FLUCTUATION DURING YEAST CELL CYCLE AND SELECTION OF SYNCHRONOUS CULTURES
PERIODIC DENSITY FLUCTUATION DURING YEAST CELL CYCLE AND SELECTION OF SYNCHRONOUS CULTURES
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DOI：
10.1128/jb.104.3.1280-1285.1970
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发表时间：
1970-01-01
影响因子：
3.2
通讯作者：
HARTWELL, LH
中科院分区：
文献类型：
--
作者：
HARTWELL, LH
酵母细胞在细胞分裂周期中经历密度的周期性波动，因此在细胞分离时密度最小，而在脱氧核糖核酸复制和核分裂之间密度最大。同步培养可以从异步生长的细胞培养中选择，提取密度最小或最大的细胞，然后在renografin -蔗糖密度梯度中条带化。这种技术快速、可重复，而且容量几乎是无限的。
Yeast cells undergo periodic fluctuations in density during the cell division cycle such that a minimum in density occurs at the time of cell separation whereas a maximum occurs between the time of deoxyribonucleic acid replication and nuclear division. Synchronous cultures can be selected from asynchronously growing cell cultures by withdrawing the cells of least or greatest density after banding in Renografin-sucrose density gradients. This technique is rapid, reproducible, and almost unlimited in capacity.