The C-reactive protein of tongue sole Cynoglossus semilaevis is an acute phase protein that interacts with bacterial pathogens and stimulates the antibacterial activity of peripheral blood leukocytes
The C-reactive protein of tongue sole Cynoglossus semilaevis is an acute phase protein that interacts with bacterial pathogens and stimulates the antibacterial activity of peripheral blood leukocytes
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DOI：
10.1016/j.fsi.2012.12.001
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发表时间：
2013-02-01
影响因子：
4.7
通讯作者：
Sun, Li
中科院分区：
文献类型：
--
作者：
Li, Mo-fei;Chen, Cheng;Sun, Li
五聚蛋白是一类进化上保守的蛋白质家族，在先天免疫中起着重要作用。C-反应蛋白（CRP）是五聚蛋白家族的成员，在人类中已知是主要的急性期蛋白。在这项工作中，我们报告的CRP，CsCRP，从半滑舌鳎（Cynoglossus semilaevis）的鉴定和分析。CsCRP由228个氨基酸残基组成，具有Pentraxin/CRP结构域。CsCRP的表达发生在广泛的组织中，并且通过肾脏、脾脏、血液，特别是肝脏中的病原体感染而上调。细菌感染后12 h内血中CsCRP水平迅速升高，约为基础水平的3.8倍。纯化的重组CsCRP（rCsCRP）能够与革兰氏阴性菌和革兰氏阳性菌，包括致病性细菌，以剂量依赖性的方式相互作用。当rCsCRP存在时，外周血白细胞（PBL）被细菌病原体感染时，细胞的呼吸爆发和吞噬能力显著增加。总之，这些结果表明，CsCRP是一种急性期蛋白，在先天免疫防御细菌感染中发挥作用。(C)2012爱思唯尔有限公司保留所有权利。
Pentraxins are a family of evolutionarily conserved proteins that play an important part in innate immunity. C-reactive protein (CRP) is a member of the pentraxin family and in humans is known to be the major acute phase protein. In this work, we report the identification and analysis of a CRP, CsCRP, from half-smooth tongue sole (Cynoglossus semilaevis). CsCRP is composed of 228 amino acid residues and possesses a Pentraxin/CRP domain. Expression of CsCRP occurred in a wide range of tissues and was upregulated by pathogen infection in kidney, spleen, blood, and, in particular, liver. Following bacterial infection, CsCRP level in blood rose rapidly within 12 h and was approximately 3.8 fold of that of the basal level. Purified recombinant CsCRP (rCsCRP) was able to interact with Gram-negative and Gram-positive bacteria including those of pathogenic nature in a dose-dependent manner. When peripheral blood leukocytes (PBL) were infected with bacterial pathogen in the presence of rCsCRP, the respiratory burst and phagocytic capacity of the cells were increased to significant extents. Taken together, these results indicate that CsCRP is an acute phase protein that plays a role in innate immune defense against bacterial infection. (C) 2012 Elsevier Ltd. All rights reserved.