iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians.
iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians.
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基于 iTRAQ 的涡虫头部再生启动定量蛋白质组学分析
DOI：
10.1371/journal.pone.0132045
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Geng X;Wang G;Qin Y;Zang X;Li P;Geng Z;Xue D;Dong Z;Ma K;Chen G;Xu C
日本三角涡虫具有惊人的能力，可以从截肢残端的前端再生出头部，并保持原有的前后极性。尽管真涡虫是一个非常有吸引力的再生分子研究系统，而且研究主要集中在转录水平上阐明真涡虫再生启动的分子机制，但真涡虫头再生（PHR）的启动机制在蛋白水平上仍不清楚。在这里，在PHR的早期阶段的蛋白质组动态的全球分析进行了使用同量异位素标签相对和绝对定量（iTRAQ）为基础的定量蛋白质组学策略，我们的数据可通过ProteomeXchange与标识符PXD 002100。结果表明，在截肢后2 h和6 h，有162个蛋白质表达差异。此外，对差异表达蛋白的表达模式和功能富集的分析表明，参与肌肉收缩、氧化还原和蛋白质合成的蛋白在PHR的启动中上调。此外，独创性途径分析表明，与细胞迁移相关的主要信号通路如ILK、钙、EIF2和mTOR信号通路，细胞增殖和蛋白质合成可能参与了PHR的启动，首次从蛋白质水平证明肌肉收缩和ILK信号在PHR的启动中起重要作用。本研究结果为进一步揭示真涡虫头部再生启动机制提供了分子基础。
The planarian Dugesia japonica has amazing ability to regenerate a head from the anterior ends of the amputated stump with maintenance of the original anterior-posterior polarity. Although planarians present an attractive system for molecular investigation of regeneration and research has focused on clarifying the molecular mechanism of regeneration initiation in planarians at transcriptional level, but the initiation mechanism of planarian head regeneration (PHR) remains unclear at the protein level. Here, a global analysis of proteome dynamics during the early stage of PHR was performed using isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics strategy, and our data are available via ProteomeXchange with identifier PXD002100. The results showed that 162 proteins were differentially expressed at 2 h and 6 h following amputation. Furthermore, the analysis of expression patterns and functional enrichment of the differentially expressed proteins showed that proteins involved in muscle contraction, oxidation reduction and protein synthesis were up-regulated in the initiation of PHR. Moreover, ingenuity pathway analysis showed that predominant signaling pathways such as ILK, calcium, EIF2 and mTOR signaling which were associated with cell migration, cell proliferation and protein synthesis were likely to be involved in the initiation of PHR. The results for the first time demonstrated that muscle contraction and ILK signaling might played important roles in the initiation of PHR at the global protein level. The findings of this research provide a molecular basis for further unraveling the mechanism of head regeneration initiation in planarians.
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