Functional evaluation of the role of reticuloendotheliosis virus long terminal repeat (LTR) integrated into the genome of a field strain of Marek's disease virus
Functional evaluation of the role of reticuloendotheliosis virus long terminal repeat (LTR) integrated into the genome of a field strain of Marek's disease virus
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DOI：
10.1016/j.virol.2009.11.017
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发表时间：
2010-02-20
期刊：
影响因子：
3.7
通讯作者：
Cui, Zhi-zhong
中科院分区：
文献类型：
--
作者：
Sun, Ai-jun;Xu, Xiao-yun;Cui, Zhi-zhong
MDV-GX 0101是一种马立克氏病病毒野毒株，具有网状内皮组织增生病病毒（REV）LTR片段的天然插入。为了研究REV-LTR插入MDV基因组的生物学特性，我们构建了MDV GX 0101株的全长感染性BAC克隆，并通过BAC突变删除了LTR序列。在感染的SPF禽中评价了LTR缺失病毒的致病特性。研究表明，LTR缺失的病毒对感染禽的生长速度具有更强的抑制作用，并诱导更强的免疫抑制作用。然而，令人惊讶的是，LTR缺失病毒的水平传播能力似乎明显弱于其亲本LTR完整病毒。尽管确切的分子机制尚不清楚，但我们的研究结果表明，REV-LTR在MDV基因组中的保留降低了其致病作用，但增加了其水平传播的可能性。(C)2009 Elsevier Inc. All rights reserved.
MDV-GX0101 is a field strain of Marek's disease virus with a naturally occurring insertion of the reticuloendotheliosis virus (REV) LTR fragment. In order to study the biological properties of REV-LTR insertion in the MDV genome, we constructed a full-length infectious BAC clone of MDV-GX0101 strain and deleted the LTR sequences by BAC mutagenesis. The pathogenic properties of the LTR-deleted virus were evaluated in infected SPF birds. The study demonstrated that the LTR-deleted virus had a stronger inhibitory effect on the growth rates of the infected birds and induced stronger immunosuppressive effects. Surprisingly, however, the ability for horizontal transmission of the LTR-deleted virus appeared to be significantly weaker than its parental LTR-intact virus. Even though the precise molecular mechanisms are still not clear, the results of our studies demonstrate that the retention of the REV-LTR in the MDV genome decreases its pathogenic effects but increases its potential for horizontal transmission. (C) 2009 Elsevier Inc. All rights reserved.