Cascading DNA Generation Reaction for Controlling DNA Nanomachines at a Physiological Temperature
Cascading DNA Generation Reaction for Controlling DNA Nanomachines at a Physiological Temperature
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DOI：
10.1007/s00354-015-0304-5
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发表时间：
2015-07
影响因子：
2.6
通讯作者：
K. Komiya;M. Yamamura
中科院分区：
文献类型：
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作者：
K. Komiya;M. Yamamura
我们开发了一个反应系统，在生理温度下生成多个单链DNA物种，以控制DNA纳米机器的运行。在这个反应系统中，级联DNA的产生是通过排列和改变模板DNA序列的组合以模块化的方式任意编程的。由于生成的DNA链与模板的解离完全依赖于DNA聚合酶的链位移活性，因此DNA链的生成和随后的杂交可以在反应温度下的一锅反应中实现。我们通过监测分子信标结构转变引起的荧光变化作为简单的DNA纳米机器操作，实验证实了DNA链在明显低于熔化温度的温度下产生和杂交。然后，我们展示了层叠DNA生成的通用性和可编程性，最多可达三层。通过将所提出的DNA生成反应与各种类型的DNA纳米机器相结合，有望实现智能分子机器人系统。
We developed a reaction system to generate multiple single-stranded DNA species at a physiological temperature for controlling the operation of DNA nanomachines. In this reaction system, cascading DNA generation is arbitrarily programmed by permutation and altering the combinations of template DNA sequences in a modular fashion. Because the dissociation of generated DNA strands from their templates is fully dependent on the strand displacement activity of DNA polymerase, generation and subsequent hybridization of DNA strands can be implemented in a one-pot reaction at the reaction temperature. We experimentally confirmed the generation and hybridization of DNA strands at a temperature remarkably lower than the melting temperature by monitoring the fluorescence change caused by the structural transition of molecular beacons as a simple DNA nanomachine operation. Then, we demonstrated the versatility and programmability of the cascading DNA generation up to three layers. By integrating the proposed DNA generation reaction with various types of DNA nanomachines, an intelligent molecular robotic system is expected to be achieved.