Expression of hepatic and ovarian cytochrome P450 during estrous cycle in rats
Expression of hepatic and ovarian cytochrome P450 during estrous cycle in rats
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大鼠动情周期肝、卵巢细胞色素P450的表达
作者：
Sang Yoon Lee;S. Oh;K. Yun;Hwan‐Mook Kim;Bong;Kiho Lee;S. K. Kim
众所周知，药物代谢的性别差异主要归因于性激素和生长激素的变化。血清中雌二醇、孕酮、催乳素、卵泡刺激素和黄体生成素的浓度在人类月经周期和大鼠发情周期中发生显著变化。然而，关于人类月经周期或大鼠发情周期对细胞色素P450（CYP1A1）亚型表达和活性的影响，目前几乎没有相关的信息。本研究旨在测定发情周期中肝脏和卵巢中CYP依赖性药物代谢酶的表达和活性。在动情间期和动情前期的雌性大鼠中，测定了肝脏和卵巢中微粒体CYP亚型（CYP1A1、CYP1A2、CYP1B1、CYP2B1、CYP2C11、CYP2C12、CYP2E1、CYP3A1、CYP3A2和CYP4A）、细胞色素b5和NADPH依赖性CYP还原酶的表达和活性。我们的结果表明，尽管动情前期血清雌二醇浓度和子宫重量显着增加，但间情期和动情前期肝脏和卵巢的CYP亚型、细胞色素b5和NADPH依赖性CYP还原酶的表达和活性没有差异。这些结果表明，细胞色素P450依赖性系统是不敏感的动情周期的变化，和进一步的研究是必要的，以确定在体内代谢外源性物质的动情周期的影响。
It is known that gender differences in drug metabolism are largely attributed to changes in sex and growth hormones. Serum concentrations of estradiol, progesterone, prolactin, follicle-stimulating hormone, and luteinizing hormone change markedly during the human menstrual cycle and the rat estrous cycle. However, little information is available regarding the effects of the human menstrual cycle or the rat estrous cycle on expression and activity of cytochrome P450 (CYP) isoforms. The present study was carried out to determine the expression and activity of CYP-dependent drug-metabolizing enzymes in the liver and ovary during the estrous cycle. The expression and activity of microsomal CYP isoforms (CYP1A1, CYP1A2, CYP1B1, CYP2B1, CYP2C11, CYP2C12, CYP2E1, CYP3A1, CYP3A2, and CYP4A), cytochrome b5 and NADPH-dependent CYP reductase in the liver and ovary were measured in female rats in diestrus and proestrus. Our results indicated that hepatic and ovarian expression and activity of CYP isoforms, cytochrome b5, and NADPH-dependent CYP reductase were not different between diestrus and proestrus, although serum estradiol concentration and uterus weight were markedly increased in the proestrus phase. These results suggest that the cytochrome P450-dependent system is not sensitive to changes in the estrous cycle, and further studies are warranted to determine the effects of the estrous cycle on in vivo metabolism of xenobiotics.
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从苯巴比妥诱导的大鼠肝脏中纯化的细胞色素 P-450 同工酶催化雄激素羟基化的区域选择性和立体选择性。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Waxman,DJ;Ko,A;Walsh,C
通讯作者：
Walsh,C
DOI：
10.1111/j.1530-0277.2001.tb02242.x
发表时间：
2001-04-01
影响因子：
3.2
作者：
Baraona, E;Abittan, CS;Lieber, CS
通讯作者：
Lieber, CS
影响因子：
--
作者：
T. Kawamoto;Satoru Kakizaki;Kouich Yoshinari;M. Negishi
通讯作者：
T. Kawamoto;Satoru Kakizaki;Kouich Yoshinari;M. Negishi
循环生长激素模式中的脉冲间隔调节肝细胞色素 P450 的性二态性表达。
DOI：
10.1073/pnas.88.15.6868
发表时间：
1991
影响因子：
11.1
作者：
Waxman,DJ;Pampori,NA;Ram,PA;Agrawal,AK;Shapiro,BH
通讯作者：
Shapiro,BH
影响因子：
3.6
作者：
D. Koop
通讯作者：
D. Koop