Establishment and characteristics of a human lung adenocarcinoma cell line.
Establishment and characteristics of a human lung adenocarcinoma cell line.
复制标题
人肺腺癌细胞系的建立及其特性。
DOI：
10.20772/cancersci1959.67.4_483
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
T. Kimoto
中科院分区：
文献类型：
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作者：
T. Akagi;T. Kimoto
从人肺腺癌的胸腔积液中建立了永久细胞系（HLC-1）。该细胞系的特点是上皮细胞单层和多层类器官生长，倍增时间约为33小时，染色体众数为68。克隆效率在液体培养基中为17.9%，在软琼脂中为8.3%。该细胞产生大量的上皮粘蛋白。电子显微镜检查发现许多分泌颗粒和末端棒。它们在旋转培养物中形成球形聚集体，在组织学上显示出腺癌样管状结构。酶组织化学显示，除葡萄糖-6-磷酸酶和ATP酶等少数酶外，它们均显示出肺腺癌细胞的特征。细胞的异种移植产生了肿瘤。这些特征证实HLC-1细胞系是人肺腺癌细胞系。
A permanent cell line (HLC-1) was established from the pleural effusion of a human lung adenocarcinoma. The cell line was characterized by the monolayered and multilayered organoid growth of epithelioid cells with the doubleing time of about 33 hr and the modal chromosome number of 68. Cloning efficiency was 17.9% in liquid medium and 8.3% in soft agar. The cell produced a large amount of epithelial mucin. Electron microscopic examination revealed many secretory granules and terminal bars. They formed spherical aggregates in a gyratory culture which showed adenocarcinoma-like tubular structures histologically. Enzyme-histolochemically, they showed the characteristics of lung adenocarcinoma cells except for a few enzymes such as glucose-6-phosphatase and ATPase. Heterotransplantation of the cells produced the tumor. These characteristics confirm that HLC-1 cell line is a human lung adenocarcinoma cell line.