Altered profile of secondary metabolites in the root exudates of Arabidopsis ATP-binding cassette transporter mutants
Altered profile of secondary metabolites in the root exudates of Arabidopsis ATP-binding cassette transporter mutants
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DOI：
10.1104/pp.107.109587
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发表时间：
2008-02-01
期刊：
影响因子：
7.4
通讯作者：
Vivanco, Jorge M.
中科院分区：
文献类型：
--
作者：
Badri, Dayakar V.;Loyola-Vargas, Victor M.;Vivanco, Jorge M.
根据最近的间接证据表明，ATP结合盒（ABC）转运蛋白在植物化学物质的根分泌中的作用，我们确定了25 ABC转运蛋白基因在根细胞中高度表达，最有可能参与分泌过程。在这25个基因中，我们还选择了6个全长ABC转运蛋白和一个半大小转运蛋白进行深入的分子和生化分析。我们比较了这七个ABC转运蛋白突变体的野生型渗出根植物化学概况。有三个非极性的植物化学物质失踪在各种ABC转运蛋白突变体相比，野生型时，样品进行了分析，通过高效液相色谱-质谱。这些数据表明，一个以上的ABC转运蛋白可以参与一个给定的植物化学物质的分泌和转运蛋白可以参与一个以上的次级代谢产物的分泌。还通过气相色谱-质谱法分析了突变体的根分泌物中存在的初级和次级代谢产物，其允许鉴定与野生型相比在一些突变体中差异发现的化合物组。例如，与野生型相比，突变体Atpdr 6分泌较低水平的有机酸，Atmrp 2分泌较高水平的氨基酸。我们的结论是，释放的植物化学物质的根是部分控制ABC转运。
Following recent indirect evidence suggesting a role for ATP-binding cassette (ABC) transporters in root exudation of phyto-chemicals, we identified 25 ABC transporter genes highly expressed in the root cells most likely to be involved in secretion processes. Of these 25 genes, we also selected six full-length ABC transporters and a half-size transporter for in-depth molecular and biochemical analyses. We compared the exuded root phytochemical profiles of these seven ABC transporter mutants to those of the wild type. There were three nonpolar phytochemicals missing in various ABC transporter mutants compared to the wild type when the samples were analyzed by high-performance liquid chromatography-mass spectrometry. These data suggest that more than one ABC transporter can be involved in the secretion of a given phytochemical and that a transporter can be involved in the secretion of more than one secondary metabolite. The primary and secondary metabolites present in the root exudates of the mutants were also analyzed by gas chromatography- mass spectrometry, which allowed for the identification of groups of compounds differentially found in some of the mutants compared to the wild type. For instance, the mutant Atpdr6 secreted a lower level of organic acids and Atmrp2 secreted a higher level of amino acids as compared to the wild type. We conclude that the release of phytochemicals by roots is partially controlled by ABC transporters.