Comprehensive Profiling of Phytohormones in Honey by Sequential Liquid-Liquid Extraction Coupled with Liquid Chromatography-Mass Spectrometry
Comprehensive Profiling of Phytohormones in Honey by Sequential Liquid-Liquid Extraction Coupled with Liquid Chromatography-Mass Spectrometry
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通过连续液-液萃取结合液相色谱-质谱法对蜂蜜中的植物激素进行全面分析
DOI：
10.1021/acs.jafc.6b04234
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发表时间：
2017
影响因子：
6.1
通讯作者：
Feng Yu-Qi
中科院分区：
文献类型：
--
作者：
Wang Qing;Cai Wen-Jing;Yu Lei;Ding Jun;Feng Yu-Qi
蜂蜜具有多种营养和药用功能，这些功能与蜂蜜中的活性成分密切相关，因此，开发蜂蜜中的新化合物具有重要意义。由于蜂蜜是花蜜的副产品，花蜜中含有丰富的植物激素，因此蜂蜜中存在植物激素是意料之中的。建立了蜂蜜中49种植物激素的连续液液萃取-液相色谱-串联质谱（LC-MS/MS）联用分析方法。49种植物激素的线性关系良好，相关系数（R）大于0.9913。方法的检出限为0.2-628.2 pg/mL。日内和日间精密度的相对标准偏差（RSD）小于15.8%，相对回收率为80.4%至123.7%，达到了满意的重现性和可靠性。该方法进一步应用于14个单花蜂蜜原料样品和3个商品蜂蜜样品中植物激素的分析。确认存在34种植物激素，包括14种细胞分裂素（CK）、8种赤霉素（GAs）、5种油菜素类固醇（BR）、吲哚-3-乙酸（IAA）、吲哚-3-丁酸（IBA）、脱落酸（ABA）、水杨酸（SA）、茉莉酸（JA）、茉莉酸-亮氨酸（JA-Leu）和茉莉酸-苯丙氨酸（JA-Phe）。此外，不同种类蜂蜜中植物激素的含量和种类也不同。该研究有助于全面阐明蜂蜜的植物激素组成，并有助于阐明蜂蜜的营养和药用作用机制。
Honey exhibits various nutritional and medicinal functions, which are highly related to the active components; thus, the exploration of new compounds in honey is of great importance. Because honey is a byproduct of flower nectar, which is rich in phytohormones, the existence of phytohormones in honey is anticipated. In this research, a method for comprehensive profiling of 49 phytohormones in honey was developed by sequential liquid–liquid extraction (LLE) coupled with liquid chromatography–tandem mass spectrometry (LC–MS/MS). Good linearities for 49 phytohormones were obtained with correlation coefficients (R) larger than 0.9913. The limits of detection (LODs) were in the range of 0.2–628.2 pg/mL. Satisfied reproducibility and reliability were achieved by evaluation of the intra- and interday precisions with relative standard deviations (RSDs) less than 15.8% and relative recoveries ranging from 80.4 to 123.7%. The method was further applied to analyze the phytohormones in 14 monofloral raw honey samples and 3 commercial honey samples. The existence of 34 phytohormones was confirmed, including 14 cytokinins (CKs), 8 gibberellins (GAs), 5 brassinosteroids (BRs), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), jasmonoyl-leucine (JA-Leu), and jasmonoyl-phenylalanine (JA-Phe). In addition, the content and species of phytohormones varies in different kinds of honey. The study is beneficial to fully illustrate the phytohormone profile of honey and contributive to elucidate the mechanism of its nutritional and medicinal functions.