cDNA and deduced protein sequence of acetylcholinesterase from the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)
cDNA and deduced protein sequence of acetylcholinesterase from the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)
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DOI：
10.1303/aez.2003.49
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发表时间：
2003-02
影响因子：
1.3
通讯作者：
Xiao-yu Ni;T. Tomita;S. Kasai;Y. Kono
中科院分区：
文献类型：
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作者：
Xiao-yu Ni;T. Tomita;S. Kasai;Y. Kono
对小菜蛾（Plutella xylostella（L.）确定了用引物步移法获得了一个2.3kb的AChE前体cDNA片段，该片段含有编码638个氨基酸残基的ORF。推导的蛋白质序列与科罗拉多马铃薯甲虫（CPB）和绿色稻叶蝉的AChE具有最高的同源性。DBM AChE属于黑腹果蝇Ace-orthopathic昆虫AChE亚家族。通过北方杂交分析检测到一个AChE基因转录本（7.6 kb）。这是首次报道鳞翅目昆虫乙酰胆碱酯酶cDNA的完整编码序列。
The sequence of acetylcholinesterase (AChE) cDNA from the diamondback moth (DBM), Plutella xylostella (L.) was determined. A 2.3 kb partial cDNA sequence, containing an ORF encoding AChE precursor with 638 amino acid residues was obtained by primer walking. The deduced protein sequence showed the highest homology to AChE of the Colorado potato beetle (CPB) and the green rice leafhopper. The DBM AChE belongs to the Drosophila melanogaster Ace-orthologous insect AChE subfamily. A single AChE gene transcript (7.6 kb) was detected by Northern blot analysis. This is the first report of the complete coding sequence of AChE cDNA from a lepidopteran insect.