Established diet-induced obesity in female rats leads to offspring hyperphagia, adiposity and insulin resistance
Established diet-induced obesity in female rats leads to offspring hyperphagia, adiposity and insulin resistance
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DOI：
10.1007/s00125-009-1316-9
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发表时间：
2009-06-01
期刊：
影响因子：
8.2
通讯作者：
Reusens, B.
中科院分区：
文献类型：
--
作者：
Nivoit, P.;Morens, C.;Reusens, B.
越来越多的证据表明，母亲肥胖可能会增加后代患代谢性疾病的风险。我们研究了建立母亲饮食诱导的肥胖对雄性和雌性后代的食欲，葡萄糖稳态和身体组成的影响。雌性Wistar大鼠在交配前和整个怀孕和哺乳期喂养标准食物（3%脂肪，7%糖[wt/wt]）或可口的致肥胖饮食（11%脂肪，43%糖[wt/wt]）8周。对照组和肥胖组母鼠的雄性和雌性后代断奶后开始进食标准食物，并进行评估，直到12个月大。在交配时，肥胖组母鼠比对照组重，并伴有高脂血症和高胰岛素血症。肥胖母鼠的雄性和雌性后代在12月龄之前都比对照组贪食（p <0.0001）和体重（p < 0.0001）。NEFA在2个月时升高，但在12个月时未升高。3个月时，OGTT显示肥胖动物雄性后代的葡萄糖稳态比雌性后代的葡萄糖稳态有更明显的改变。在雌性8 - 9个月和雄性10 - 11个月时进行的正常血-高胰岛素钳夹试验显示，肥胖母鼠的雄性后代存在胰岛素抵抗（与对照组相比，p < 0.05）。12个月时的身体成分分析也显示肥胖动物的雄性和雌性后代的脂肪垫重量增加。雌性大鼠的饮食诱导的肥胖导致雄性后代的胰岛素抵抗状态，与肥胖和肥胖增加的发展有关。食物摄入量的增加可能起作用。
Accumulating evidence suggests that maternal obesity may increase the risk of metabolic disease in the offspring. We investigated the effects of established maternal diet-induced obesity on male and female offspring appetite, glucose homeostasis and body composition in rats.Female Wistar rats were fed either a standard chow (3% fat, 7% sugar [wt/wt]) or a palatable obesogenic diet (11% fat, 43% sugar [wt/wt]) for 8 weeks before mating and throughout pregnancy and lactation. Male and female offspring of control and obese dams were weaned on to standard chow and assessed until 12 months of age.At mating, obese dams were heavier than control with associated hyperglycaemia and hyperinsulinaemia. Male and female offspring of obese dams were hyperphagic (p < 0.0001) and heavier than control (p < 0.0001) until 12 months of age. NEFA were raised at 2 months but not at 12 months. At 3 months, OGTT showed more pronounced alteration of glucose homeostasis in male than in female offspring of obese animals. Euglycaemic-hyperinsulinaemic clamps performed at 8 to 9 months in female and 10 to 11 months in male offspring revealed insulin resistance in male offspring of obese dams (p < 0.05 compared with control). Body compositional analysis at 12 months also showed increased fat pad weights in male and female offspring of obese animals.Diet-induced obesity in female rats leads to a state of insulin resistance in male offspring, associated with development of obesity and increased adiposity. An increase in food intake may play a role.