Inhibition of lactose-reversible adherence between Actinomyces viscosus and oral streptococci by salivary components.
Inhibition of lactose-reversible adherence between Actinomyces viscosus and oral streptococci by salivary components.
复制标题
唾液成分抑制粘性放线菌和口腔链球菌之间的乳糖可逆粘附。
DOI：
10.1159/000260765
复制
发表时间：
1984
期刊：
影响因子：
4.2
通讯作者：
Gibbons,RJ
中科院分区：
文献类型：
--
作者：
Komiyama,K;Gibbons,RJ
研究了唾液对粘性放线菌与血链球菌或混合链球菌之间乳糖可逆黏附的影响。因为唾液凝集了这些微生物，所以有必要建立模型来研究链球菌对放线菌细胞的附着，而不是依赖于两个相互作用的生物体的混合凝集。一个模型涉及放射性标记的链球菌与涂有放线菌的精胺偶联琼脂糖珠的结合。这个测试似乎与之前描述的共聚集反应是平行的，因为共聚集阳性的链球菌菌株附着在放线菌包被的珠子上的数量比共聚集阴性菌株要多得多。乳糖和已经耗尽链球菌凝集素的唾液强烈地抑制共聚集阳性生物体的附着。第二个模型涉及使用放线菌细胞的声波提取物，它可以凝集共凝集阳性的链球菌菌株，但不凝集共聚阴性的菌株。这种提取物诱导的链球菌凝集也被乳糖和从代表A、B和O血型的6名献血者的唾液样本所抑制。
The influence of saliva on lactose-reversible adherence between strains ofActinomyces viscosusandStreptococcus sanguisorStreptococcus mitiswas investigated. Because saliva agglutinated these organisms, it was necessary to develop models for studying streptococcal attachment toActinomycescells which did not depend upon mixed agglutination of both interacting organisms. One model involved the binding of radiolabelled streptococci to spermine-conjugated agarose beads coated withActinomyces. This assay appeared to parallel previously described coaggregation reactions because coaggregation-positive streptococcal strains attached in much higher numbers to theActinomyces-coated beads than did coaggregation-negative strains. Lactose and also saliva which had been depleted of streptococcal agglutinins strongly inhibited attachment of coaggregation-positive organisms. A second model involved use of sonic extracts ofActinomycescells which agglutinated coaggregation-positive streptococcal strains, but not coaggregation-negative strains. This extract-induced streptococcal agglutination was also inhibited by lactose and by saliva samples collected from 6 donors representative of blood types A, B, and O.