Mouse models of infertility due to swollen spermatozoa
Mouse models of infertility due to swollen spermatozoa
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DOI：
10.1016/j.mce.2003.10.076
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发表时间：
2004-03-15
影响因子：
4.1
通讯作者：
Sipilä, P
中科院分区：
文献类型：
--
作者：
Cooper, TG;Yeung, CH;Sipilä, P
比较了患有男性不育的转基因小鼠、c-ros 敲除 (KO) 和 GPX5-Tag2 转基因小鼠模型。两者都表现出严重成角的精子鞭毛，这解释了不育症。由于有角的精子是肿胀的细胞，因此表明体积调节失败。还发现了基因型之间的差异：c-ros KO 的尾部精子可以在体外使卵子受精，而 GPX5-Tag2 则不行；与 c-ros KO 小鼠相比，GPX5-Tag2 的附睾内鞭毛成角更多发生；仅GPX5-Tag2小鼠附睾尾部液体渗透压较低； c-ros KO 小鼠（而非 GPX5-Tag2 小鼠）尾部精子的角度在脱膜后减少。这些观察结果表明 GPX5-Tag2 小鼠表现出更早、更严重的缺陷。附睾基因芯片分析显示，两种基因型中 CRES（胱抑素相关附睾生精）和 MEP17（鼠附睾蛋白 17）基因的表达均降低。进一步的分析可以查明附睾调节精子体积所必需的基因，解释不孕症并提出男性避孕方式。 (C) 2003 Elsevier Ireland Ltd. 保留所有权利。
Transgenic mice with male infertility, the c-ros knockout (KO) and GPX5-Tag2 transgenic mouse models, are compared. Both exhibit severely angulated sperm flagella explaining the infertility. As angulated spermatozoa are swollen cells, a failure in volume regulation is indicated. Differences between genotypes were also found: caudal spermatozoa from c-ros KO, but not GPX5-Tag2, could fertilise eggs in vitro; flagellar angulation occurred more within the epididymis of GPX5-Tag2 than c-ros KO mice; the osmotic pressure of cauda epididymidal fluid was lower only in GPX5-Tag2 mice; angulation of caudal sperm from c-ros KO, but not GPX5-Tag2 mice, decreased upon demembranation. These observations indicate that GPX5-Tag2 mice express an earlier, more severe defect. Gene chip analyses of the epididymides revealed decreased expression of the CRES (cystatin-related epididymal-spermatogenic) and MEP17 (murine epididymal protein 17) genes in both genotypes. Further analysis could pinpoint genes essential for epididymal regulation of sperm volume, explain infertility and suggest modes of male contraception. (C) 2003 Elsevier Ireland Ltd. All rights reserved.