Imaging Viral Behavior in Mammalian Cells with Self-Assembled Capsid-Quantum-Dot Hybrid Particles
Imaging Viral Behavior in Mammalian Cells with Self-Assembled Capsid-Quantum-Dot Hybrid Particles
复制标题
利用自组装衣壳-量子点混合颗粒对哺乳动物细胞中的病毒行为进行成像
DOI：
10.1002/smll.200801303
复制
发表时间：
2009-03-20
期刊：
影响因子：
13.3
通讯作者：
Zhang, Xian-En
中科院分区：
文献类型：
--
作者：
Li, Feng;Zhang, Zhi-Ping;Zhang, Xian-En
量子点（QDs）独特的光谱特性使超灵敏和长期的生物标记成为可能。为了追踪病毒在活细胞中的感染、运动和定位，通过体外自组装猿猴病毒40 （SV40）主要衣壳蛋白，构建了含有qd的病毒样颗粒（VLPs），称为SVLP-QDs。SVLP-QDs在尺寸上具有均匀性（约为24 nm），光谱性质与未封装的QDs相似，并且具有相当的稳定性。当与活细胞孵育时，SVLP-QDs显示通过腔泡内吞作用进入细胞，沿着微管移动，并在内质网中积累。这个过程模拟了SV40的早期感染步骤。这是在活细胞中用封装量子点成像病毒行为的第一个范例。该方法可为各种目的提供新的替代方法，如追踪病毒或病毒组分、靶向纳米颗粒递送和探测药物递送。
Unique spectral properties of quantum dots (QDs) enable ultrasensitive and long-term biolabeling. Aiming to trace the infection, movement, and localization of viruses in living cells, QD-containing virus-like particles (VLPs) of simian virus 40 (SV40), termed SVLP-QDs, are constructed by in vitro self-assembly of the major capsid protein of SV40. SVLP-QDs show homogeneity in size (approximate to 24 nm), similarity in spectral properties to unencapsidated QDs, and considerable stability. When incubated with living cells, SVLP-QDs are shown to enter the cells by caveolar endocytosis, travel along the microtubules, and accumulate in the endoplasmic reticulum. This process mimics the early infection steps of SV40. This is the first paradigm of imaging viral behaviors with encapsidated QDs in living cells. The method may provide a new alternative for various purposes, such as tracing viruses or viral components, targeted nanoparticle delivery, and probing of drug delivery.