Expression of Axin2 Indicates a Role for Canonical Wnt Signaling in Development of the Crown and Root During Pre- and Postnatal Tooth Development
Expression of Axin2 Indicates a Role for Canonical Wnt Signaling in Development of the Crown and Root During Pre- and Postnatal Tooth Development
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DOI：
10.1002/dvdy.22047
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发表时间：
2010-01-01
影响因子：
2.5
通讯作者：
Sharpe, Paul T.
中科院分区：
文献类型：
--
作者：
Lohi, Mahtab;Tucker, Abigail S.;Sharpe, Paul T.
此前，两只报告小鼠 TOPgal 和 BATgal 已被用来揭示典型 Wnt 活性的空间模式，直至牙齿发育的钟形阶段。为了进一步了解该通路的功能，不仅在牙发生的早期发育阶段，而且在出生后的牙齿中，我们使用了 Axin2-lacZ 小鼠，它是典型 Wnt 活性的直接报告者。随着牙齿发育的进展，Axin2 表达局限于初级和次级牙釉质结以及下面的间充质。在出生后的牙齿中，在发育中的成牙本质细胞、牙髓中观察到 Axin2 表达，并集中在发育中的牙根周围。表达被排除在成釉细胞之外，并且与磨牙尖尖处的无牙釉质区域相关。该表达确定了 Wnt 信号在定义牙釉质形成区域以及控制牙齿发育后期牙根发育方面的新作用。发展动力学 239:160-167, 2010。(C) 2009 Wiley-Liss, Inc.
Previously two reporter mice, TOPgal and BATgal, have been used to uncover the spatial patterns of canonical Wnt activity up to the bell stage of tooth development. To further understand the function of this pathway, not only at the early developmental stages of odontogenesis but also in postnatal teeth, we have used Axin2-lacZ mice a direct reporter of canonical Wnt activity. As tooth development progresses, Axin2 expression becomes localized to the primary and secondary enamel knots, and the underlying mesenchyme. In postnatal teeth, Axin2 expression is observed in developing odontoblasts, in the dental pulp and concentrated around the developing roots. Expression is excluded from the ameloblasts and associated with the enamel-free zones at the tip of the molar cusps. This expression identifies new roles for Wnt signaling in defining the regions where enamel will form, and controlling root development at late stages of tooth development. Developmental Dynamics 239:160-167, 2010. (C) 2009 Wiley-Liss, Inc.