The effect of thrombocytopenia on dermal wound healing
The effect of thrombocytopenia on dermal wound healing
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DOI：
10.1046/j.1523-1747.2003.12253.x
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发表时间：
2003-06-01
影响因子：
6.5
通讯作者：
DiPietro, LA
中科院分区：
文献类型：
--
作者：
Szpaderska, AM;Egozi, EI;DiPietro, LA
血小板在伤口中的立即出现和血小板释放生长因子的能力表明，血小板是组织修复过程的重要触发因素。为了检查全身性血小板减少症对伤口愈合的炎症和增殖方面的影响，通过腹膜内给予兔抗小鼠血小板血清使成年小鼠血小板减少。制备全层切除皮肤伤口，并在损伤后多个时间点分析炎性细胞含量、生长因子产生、上皮再生、胶原合成和血管生成。与对照小鼠相比，血小板减少的小鼠表现出显著改变的伤口炎症。血小板减少小鼠的伤口含有显著更多的巨噬细胞和T细胞，但表现出与对照小鼠伤口相似的中性粒细胞含量。令人惊讶的是，血小板减少的小鼠在伤口愈合的修复方面没有表现出延迟。创伤再上皮化、胶原蛋白合成和血管生成的速率对于血小板减少症小鼠和对照小鼠几乎相同。血管内皮生长因子，成纤维细胞生长因子2，转化生长因子β 1，角质形成细胞生长因子和表皮生长因子的分析显示，这些生长因子的水平在对照组和血小板减少小鼠的伤口中没有差异。总之，结果表明，血小板的存在可能会影响伤口炎症，但血小板不会显着影响修复的增殖方面，包括伤口闭合，血管生成和胶原蛋白合成。
The immediate appearance of platelets in wounds and the ability of platelets to release growth factors suggest that platelets are an important trigger of the tissue repair process. To examine the effect of systemic thrombocytopenia on both the inflammatory and proliferative aspects of wound healing, adult mice were rendered thrombocytopenic by intraperitoneal administration of a rabbit antimouse platelet serum. Full-thickness excisional dermal wounds were prepared and analyzed for inflammatory cell content, growth factor production, reepithelialization, collagen synthesis, and angiogenesis at multiple time points after injury. Compared to control mice, thrombocytopenic mice exhibited significantly altered wound inflammation. Wounds of thrombocytopenic mice contained significantly more macrophages and T cells, yet exhibited neutrophil content similar to wounds from control mice. Surprisingly, thrombocytopenic mice exhibited no delay in the reparative aspects of wound healing. The rate of wound reepithelialization, collagen synthesis, and angiogenesis was nearly identical for thrombocytopenic and control mice. Analysis of vascular endothelial growth factor, fibroblast growth factor 2, transforming growth factor beta1, keratinocyte growth factor, and epidermal growth factor revealed no difference in the levels of these growth factors in the wounds of control and thrombocytopenic mice. Taken together, the results suggest that the presence of platelets may influence wound inflammation, but that platelets do not significantly affect the proliferative aspects of repair, including wound closure, angiogenesis, and collagen synthesis.