Comparative Transcriptome Analysis of Resistant and Susceptible Tomato Lines in Response to Infection by Xanthomonas perforans Race T3
Comparative Transcriptome Analysis of Resistant and Susceptible Tomato Lines in Response to Infection by Xanthomonas perforans Race T3
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抗性和敏感番茄品系对穿孔黄单胞菌 T3 种感染反应的比较转录组分析
DOI：
10.3389/fpls.2015.01173
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发表时间：
2015-12-24
影响因子：
5.6
通讯作者：
Yang, Wencai
中科院分区：
文献类型：
--
作者：
Du, Heshan;Wang, Yuqing;Yang, Wencai
由多种黄单胞菌引起的细菌斑病是番茄（Solanum lycopersicum L.）的一种严重病害。尽管抗性遗传学已被广泛研究，但病原体与番茄植株之间的相互作用仍不清楚。在这项研究中，将穿孔 X. perforans T3 种感染番茄品系的转录组与对照的转录组进行了比较。使用 RNA 测序技术，在接种后 6 小时（6 HPI）和 6 天（6 DPI）时，每个样本中映射了大约 21,526 个基因，平均生成 700 万个读数。总体而言，抗性番茄品系 PI 114490 中的差异表达基因 (DEG) 数量高于易感品系 OH 88119，且 6 DPI 时的 DEG 数量高于 6 HPI 时的 DEG 数量。较少的基因（PI 114490 中的 78 个和 OH 88119 中的 15 个）上调，大多数 DEG 下调，表明诱导防御反应可能在 6 HPI 时尚未完全激活。两个番茄品系中 6 DPI 时 326 个共调节基因的累积表达水平可能与基础防御有关，而 6 DPI 时的特异性和强诱导基因可能与 PI 114490 中的抗性相关。大多数 DEG 参与植物激素信号转导、植物与病原体相互作用和苯丙氨酸代谢，PI 114490 在 6 DPI 中显着上调的基因与防御反应相关途径。还鉴定出含有 NBS-LRR 结构域或防御相关 WRKY 转录因子的 DEG。这些结果将为了解穿孔穿孔菌和番茄植株之间的相互作用提供宝贵的资源。
Bacterial spot, incited by several Xanthomonas sp., is a serious disease in tomato (Solanum lycopersicum L.). Although genetics of resistance has been widely investigated, the interactions between the pathogen and tomato plants remain unclear. In this study, tanscriptomes of X. perforans race T3 infected tomato lines were compared to those of controls. An average of 7 million reads were generated with approximately 21,526 genes mapped in each sample post-inoculation at 6 h (6 HPI) and 6 days (6 DPI) using RNA-sequencing technology. Overall, the numbers of differentially expressed genes (DEGs) were higher in the resistant tomato line PI 114490 than in the susceptible line OH 88119, and the numbers of DEGs were higher at 6 DPI than at 6 HPI. Fewer genes (78 in PI 114490 and 15 in OH 88119) were up-regulated and most DEGs were down-regulated, suggesting that the inducible defense response might not be fully activated at 6 HPI. Accumulation expression levels of 326 co-up regulated genes in both tomato lines at 6 DPI might be involved in basal defense, while the specific and strongly induced genes at 6 DPI might be correlated with the resistance in PI 114490. Most DEGs were involved in plant hormone signal transduction, plant-pathogen interaction and phenylalanine metabolism, and the genes significantly up-regulated in PI 114490 at 6 DPI were associated with defense response pathways. DEGs containing NBS-LRR domain or defense-related WRKY transcription factors were also identified. The results will provide a valuable resource for understanding the interactions between X. perforans and tomato plants.