The multifunctional role of the immunohistochemical expression of MMP-7 in invasive breast cancer
The multifunctional role of the immunohistochemical expression of MMP-7 in invasive breast cancer
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DOI：
10.1111/j.1600-0463.2005.apm_02.x
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发表时间：
2005-04-01
期刊：
影响因子：
2.8
通讯作者：
Nakopoulou, L
中科院分区：
文献类型：
--
作者：
Mylona, E;Kapranou, A;Nakopoulou, L
Mylona E，Kapranou E，Mavromanti J，Markaki S，Keramopoulos A，Nakopoulou L. MMP-7在浸润性乳腺癌中的多功能表达APMIS 2005; 113：246- 55。基质金属蛋白酶（MMPs）的分泌在癌细胞的转移中至关重要，因为MMPs负责细胞外基质（ECM）的降解。其中，基质金属蛋白酶-7（MMP-7）或基质溶解素1（matrilysin 1）是降解IV型胶原、纤连蛋白和层粘连蛋白的基质溶解素。应用免疫组化法检测MMP-7蛋白在浸润性乳腺癌中的表达。MMP-7与临床病理参数、无病生存期和总生存期、p53、c-erbB-2、topoIIa、MMP-2、uPAR和β-连环蛋白一起沿着进行研究。MMP-7在癌细胞和癌旁正常上皮细胞胞浆中阳性表达率分别为54.2%（96/177）和47.5%（84/177）。癌细胞中的MMP-7反应性与核分级（p = 0.049）和拓扑IIa（p=0.03）呈负相关。MMP-7在恶性肿瘤和间质细胞中的表达与癌细胞中的uPAR之间观察到平行关联（分别为p=0.033和p=0.027）。肿瘤间质细胞的MMP-7与相同细胞类型的MMP-2平行相关（p=0.044），而异常的P-连环蛋白表达与癌细胞的MMP-7呈负相关（p=0.047）。我们的研究结果显示了MMP-7在乳腺中的多功能作用，因为它似乎与侵袭性较低的表型相关，同时，通过与侵袭指标的合作参与侵袭。
Mylona E, Kapranou E, Mavrommatis J, Markaki S, Keramopoulos A, Nakopoulou L. The multifunctional role of the immunohistochemical expression of MMP-7 in invasive breast cancer. APMIS 2005; 113:246-55.The secretion of matrix metalloproteinases (MMPs) is crucial in the metastasis of cancer cells, since MMPs are responsible for the degradation of extracellular matrix (ECM). Among them, matrix metalloproteinase-7 (MMP-7) or matrilysin 1 is a stromelysin which degrades type-IV collagen, fibronectin and laminin. Immunohistochernistry was performed to detect MMP-7 protein in infiltrative breast carcinomas. MMP-7 was studied along with clinicopathological parameters, disease-free and overall survival, and p53, c-erbB-2, topoIIa, MMP-2, uPAR and P-catenin. MMP-7 immunoreactivity was detected in the cytoplasm of cancer cells in 54.2% (96/177) and tumor stromal cells in 47.5%, (84/177), as well as in normal epithelium adjacent to malignant epithelium. MMP-7 reactivity in cancer cells displayed an inverse association with nuclear grade (p = 0.049) and topoIIa (p=0.03). A parallel association was observed between the expression of MMP-7 in both malignant and stromal cells with uPAR in cancer cells (p=0.033 and p=0.027, respectively). MMP-7 of tumor stromal cells depicted a parallel correlation with MMP-2 of the same cell type (p=0.044), while abnormal P-catenin expression was inversely associated with MMP-7 of cancer cells (p=0.047). Our results show the multifunctional role of MMP-7 in the mammary gland, since it seems to be associated with a less aggressive phenotype, while, at the same time, being involved in invasion, through its collaboration with indicators of invasion.