The Drosophila rhomboid gene mediates the localized formation of wing veins and interacts genetically with components of the EGF-R signaling pathway.
The Drosophila rhomboid gene mediates the localized formation of wing veins and interacts genetically with components of the EGF-R signaling pathway.
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DOI：
10.1101/gad.7.6.961
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发表时间：
1993-06
影响因子：
10.5
通讯作者：
M. Sturtevant;M. Roark;E. Bier
中科院分区：
文献类型：
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作者：
M. Sturtevant;M. Roark;E. Bier
菱形 (rho) 基因编码跨膜蛋白，是果蝇胚胎中腹侧表皮分化所需的一小群基因（腹外侧基因）的成员。腹外侧基因包括编码 EGF 样配体的 spitz 和 Star。斯皮茨的受体可能是编码果蝇表皮生长因子受体（Egf-r）的基因，因为Egf-r部分功能丧失导致的表型与腹外侧群突变体相似。在编码细胞表面或分泌蛋白的腹外侧基因中，rho 是唯一以与需要腹外侧通路活性的细胞相对应的局部模式表达的成员。在本文中，我们提供的证据表明 rho 的空间定位在建立成虫翅膀中的静脉模式中起着类似的作用。 rho 在可能是翼静脉原基的早期翼盘细胞中表达，后来明显限制于发育中的静脉。具有活性 rho(ve) 等位基因的纯合果蝇具有缺失的静脉，并且 rho 无法在 rho(ve) 突变体翼盘中表达。翅膀发育过程中 rho 的异位表达导致额外静脉的形成。腹外侧基因的基因剂量研究表明，rho 产物 (Rho) 可能促进 Spi-EGF-R 信号传导，从而激活 RAS。我们讨论了 Rho 的局部表达如何放大由普遍分布的配体和受体成分介导的信号传导的模型。
The rhomboid (rho) gene, which encodes a transmembrane protein, is a member of a small group of genes (ventrolateral genes) required for the differentiation of ventral epidermis in the Drosophila embryo. The ventrolateral genes include spitz, which encodes an EGF-like ligand, and Star. The receptor for spitz may be the gene encoding the Drosophila epidermal growth factor-receptor (Egf-r) because the phenotype resulting from partial loss of function of Egf-r is similar to that of ventrolateral group mutants. Among ventrolateral genes encoding cell-surface or secreted proteins, rho is the only member expressed in a localized pattern corresponding to cells requiring the activity of the ventrolateral pathway. In this paper we provide evidence that spatial localization of rho plays an analogous role in establishing vein pattern in the adult wing. rho is expressed in early wing disc cells likely to be wing vein primordia and later is sharply restricted to developing veins. Flies homozygous for the viable rho(ve) allele have missing veins and rho fails to be expressed in rho(ve) mutant wing discs. Ectopic expression of rho during wing development leads to the formation of extra veins. Gene dosage studies among ventrolateral genes suggest that the rho product (Rho) may facilitate Spi-EGF-R signaling, resulting in activation of RAS. We discuss models for how localized expression of Rho may amplify signaling mediated by ubiquitously distributed ligand and receptor components.