Nephrotoxicity and highly active antiretroviral therapy: Mitigating action of Momordica charantia
Nephrotoxicity and highly active antiretroviral therapy: Mitigating action of Momordica charantia
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DOI：
10.1016/j.toxrep.2018.09.003
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发表时间：
2018-01-01
影响因子：
--
通讯作者：
Azu, Onyemaechi Okpara
中科院分区：
文献类型：
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作者：
Offor, Ugochukwu;Naidu, Edwin Coleridge;Azu, Onyemaechi Okpara
苦瓜（M. charantia）以其抗氧化和抗糖尿病的特性而闻名。本研究的目的是研究高活性抗逆转录病毒治疗（HAART）方案triplavar治疗大鼠后，charantia的肾保护作用。体重178.1 ~ 220.5 g的成年雄性Sprague-Dawley大鼠（n = 36）分为6组（A-F），每组6只（n = 6）。药物及提取物经灌胃给药。使用人体治疗剂量当量对大鼠模型调整三铂的治疗剂量。动物在第10周被安乐死，取肾检查，并通过心脏穿刺取血。氧化应激酶（超氧化物歧化酶- sod，过氧化氢酶- cat和还原性谷胱甘肽- gsh）水平在所有未接受M. charantia的组均显著降低。硫代巴比妥酸反应物质（TBARS）的水平增加，导致自由基通过自动氧化形成。各组肾脏指标无蛋白尿，尿素氮（BUN）、肌酐（SCr）、电解质正常。HAART治疗组（B组）出现严重的蛋白尿，BUN和SCr显著升高（p < 0.05），总电解质紊乱（p < 0.05）。未注射沙氏分枝杆菌佐剂组血糖水平显著升高（p < 0.05）。HAART治疗动物的组织病理学显示肾小球毛细血管异常和细胞浸润，而M. charantia治疗动物的肾小球外观基本正常，毛细血管袢和细胞结构正常。结论：给药可改善血糖水平，恢复肾脏组织，恢复肾功能，减轻体重，恢复高血糖。
Momordica charantia (M. charantia) is known for its antioxidant and antidiabetic properties. The aim of this study is to investigate the renoprotective effects of M. charantia in rats following treatment with highly active antiretroviral therapy (HAART) regimen triplavar. Adult male Sprague-Dawley rats weighing 178.1-220.5 g (n = 36) were divided into six groups (A-F) with each group comprising of six (n = 6) rats. The drugs and extract were administered via oral gavage. The therapeutic dose of triplavar was adjusted using the human therapeutic dose equivalent for the rat model. Animals were euthanized on the tenth week with kidneys removed for examination and blood obtained via cardiac puncture. Levels of oxidative stress enzymes (superoxide dismutase-SOD, catalase-CAT, and reduced glutathione-GSH) were significantly lowered in all groups not receiving M. charantia. The levels of thiobarbituric acid reactive substances (TBARS) were increased resulting in free radical formation via auto-oxidation. Renal parameters showed no albuminuria, normal blood urea nitrogen (BUN), serum creatinine (SCr) and electrolytes in groups treated with M. charantia. HAART treated (Group B) showed severe albuminuria, a significantly (p < 0.05) raised BUN and SCr and gross electrolyte disturbances. Blood glucose levels were significantly raised in groups not receiving the adjuvant M. charantia (p < 0.05). Histopathology in HAART treated animals showed glomerular capillary abnormalities and cellular infiltrations while M. charantia treated animals showed an essentially normal glomerular appearance with capillary loops and normal cytoarchitecture. In conclusion M. charantia extract administration improved blood glucose levels, restored renal histology, reinstate renal function, reduce body weight loss and restores hyperglycemia.