Biomimetic chitosan-calcium phosphate composites with potential applications as bone substitutes: preparation and characterization.
Biomimetic chitosan-calcium phosphate composites with potential applications as bone substitutes: preparation and characterization.
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仿生壳聚糖-磷酸钙复合材料作为骨替代品的潜在应用：制备和表征。
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
L. Verestiuc
中科院分区：
文献类型：
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作者：
C. Tanase;Marcel I Popa;L. Verestiuc
提出了一种制备壳聚糖-磷酸钙（Cs- cp）支架的新型仿生技术：在生理条件下（人体体温和体液pH分别为37℃和pH = 7.2），从其前体CaCl(2)和NaH(2) PO(4)在Cs基质上沉淀磷酸钙。通过元素分析、傅里叶变换红外光谱（FTIR）、x射线衍射（XRD）、能量色散x射线光谱（EDX）和扫描电子显微镜（SEM）等多种技术证实了材料的组成和结构。红外光谱（FTIR）和扫描电镜（SEM）数据表明，磷酸钙-羟基磷灰石（CP-Hap）排列在Cs基体上。在这种情况下，聚合物就像胶水一样，把磷酸钙晶体粘在一起。生物模拟流体（磷酸盐缓冲液- pbs和pbs -白蛋白）中的行为揭示了-NH3（+）和Ca（2+）之间的螯合作用对支架与水介质相互作用的重要贡献；复合材料中增加的壳聚糖量允许与人白蛋白相互作用并改善液体潴留。该复合材料被溶菌酶轻微降解，这有利于骨替代品的体内降解控制。弹性模量强烈依赖于壳聚糖/磷酸钙的比例，并推荐获得的仿生复合材料作为有前景的骨应用材料。
A novel biomimetic technique for obtaining chitosan-calcium phosphates (Cs-CP) scaffolds are presented: calcium phosphates are precipitated from its precursors, CaCl(2) and NaH(2) PO(4) on the Cs matrix, under physiological conditions (human body temperature and body fluid pH; 37°C and pH = 7.2, respectively). Materials composition and structure have been confirmed by various techniques: elemental analysis, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), and scanning electron microscopy (SEM). FTIR and SEM data have shown the arrangement of the calcium phosphates-hydroxyapatite (CP-Hap) onto Cs matrix. In this case the polymer is acting as glue, bonding the calcium phosphates crystals. Behavior in biological simulated fluids (phosphate buffer solution-PBS and PBS-albumin) revealed an important contribution of the chelation between -NH3(+) and Ca(2+) on the scaffold interaction with aqueous mediums; increased quantities of chitosan in composites permit the interaction with human albumin and improve the retention of fluid. The composites are slightly degraded by the lysozyme which facilitates an in vivo degradation control of bone substitutes. Modulus of elasticity is strongly dependent of the ratio chitosan/calcium phosphates and recommends the obtained biomimetic composites as promising materials for a prospective bone application.