Investigation of cuticular hydrocarbons for determining the age and survivorship of Australasian mosquitoes
Investigation of cuticular hydrocarbons for determining the age and survivorship of Australasian mosquitoes
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DOI：
10.4269/ajtmh.2006.74.462
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发表时间：
2006-03-01
影响因子：
3.3
通讯作者：
Ryan, PA
中科院分区：
文献类型：
--
作者：
Hugo, LE;Kay, BH;Ryan, PA
对实验室饲养的埃及伊蚊（Aedes aegypti（L.））进行了年龄分级蚊子表皮碳氢化合物（CH）技术的评价，法氏按蚊（Laveran）和治安黄按蚊（Skuse）。采用气相色谱/质谱法测定了不同蚊腿正己烷提取物中5种烷烃的相对丰度。从An. farauti和Ae.埃及伊蚊，它们分别是疟疾和登革热的传播媒介。女安。法劳蒂人分为两个年龄组（1-5岁和！5日龄27 ℃）和Ae.根据这些变化，将埃及伊蚊分为三个年龄组（在27摄氏度下1至< 5，5至< 9 and >= 9天）。然而，没有可预测的年龄相关的变化，碳氢化合物丰度在OC。义警模拟建模被用来构建序贯抽样准则的应用，这种技术来估计Ae的生存。aegypti和An. farauti人口。这些指南定义了存活率、采样蚊子数量、基于CH的年龄预测和存活率估计的准确性之间的关系。例如，他们证明，如果19%的Ae。通过CH分析估计埃及伊蚊>= 9天龄，指数模型的每日存活率估计值应基于200只蚊子的样本，以使存活率估计值在实际存活率的5%以内。然而，如果估计只有10%的种群>= 9天龄，则需要分析500只蚊子，以使存活率估计值具有同等精度。
The cuticular hydrocarbon (CH) technique of age grading mosquitoes was evaluated for use on laboratory-reared Aedes aegypti (L.), Anopheles farauti (Laveran), and Ochlerotatus vigilax (Skuse). Gas chromatography/mass spectroscopy was used to determine the relative abundance of five alkanes in hexane extracts from the legs of individual mosquitoes. Age-related changes to relative hydrocarbon abundances were observed from An. farauti and Ae. aegypti, which are vectors of malaria and dengue, respectively. Female An. farauti were classified into two age categories (1-5 and ! 5 days old at 27 degrees C) and Ae. aegypti into three age categories (1 to < 5, 5 to < 9 and >= 9 days old at 27 degrees C) based on these changes. However, there was an absence of predicable age-related changes to hydrocarbon abundance in Oc. vigilax. Simulation modeling was used to construct sequential sampling guidelines for the application of this technique to estimate the survivorship of Ae. aegypti and An. farauti populations. These guidelines define the relationship between the survival rate, number of mosquitoes sampled, CH-based predictions of age, and the accuracy of survival rate estimates. They demonstrated, for example, that if 19% of a population of Ae. aegypti is estimated to be >= 9 days old by CH analysis, an estimate of the daily survival rate from the exponential model should be based on a sample of 200 mosquitoes for the survival rate estimate to be within 5% of the actual rate. However, if only 10% of the population is estimated to be >= 9 days old, 500 mosquitoes would need to be analyzed for the survival rate estimate to be of equivalent accuracy.