T cell receptor V beta gene expression in experimental herpes stromal keratitis.
T cell receptor V beta gene expression in experimental herpes stromal keratitis.
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实验性疱疹基质角膜炎中 T 细胞受体 V β 基因的表达。
DOI：
10.1038/eye.1995.147
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Foster,CS
中科院分区：
文献类型：
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作者：
Pedroza-Seres,M;Goei,S;Merayo-Lloves,J;Dutt,JE;Lee,SJ;Arrunategui-Correa,V;Foster,CS
本研究检测了实验性单纯疱疹性角膜炎（HSK）小鼠模型中T细胞受体（TCR） Vβ mRNA的表达。采用聚合酶链反应（PCR）技术检测单纯疱疹病毒1型（HSV-1）在角膜接种hsk易感小鼠和hsk抗性小鼠不同时间点眼组织中TCR Vβ mRNA的表达，并进行Southern印迹和密度分析。在hsk敏感的C. AL-20小鼠的眼睛中，与hsk耐药的CB-17小鼠相比，检测到更多样化的TCR Vβ转录物使用模式。接种后第11、14和21天，两株小鼠均表达了Vβ8家族成员。密度测定，第11天，Vβ8表达强度。2和Vβ8。C. AL-20小鼠眼中的3条信息显著增加；Vβ8。1只在CB-17小鼠中表达。hsk易感小鼠和hsk耐药小鼠的TCR Vβ表达有明显差异。两株菌株之间Vβ8家族成员表达的信息强度的差异可能与先前的实验显示Vβ8有关。HSV-1攻毒后第11天和第14天，hsk易感小鼠角膜主要浸润细胞为1,2 + T细胞。
Our study examined T cell receptor (TCR) Vβ mRNA expression in a murine model of experimental herpes simplex keratitis (HSK). We employed a polymerase chain reaction (PCR) technique to detect TCR Vβ mRNA expression in the inoculated eyes of both HSK-susceptible and HSK-resistant mice at different time points after corneal inoculation with herpes simplex virus type 1 (HSV-1), followed by Southern blotting and densitometry analysis. In eyes from HSK-susceptible C. AL-20 mice, a more diverse TCR Vβ transcript usage pattern was detected as compared with that seen in HSK-resistant CB-17 mice. Vβ8 family members were expressed in both strains of mice at days 11, 14 and 21 post-inoculation. By densitometry, at day 11, the intensity of expression of Vβ8. 2 and Vβ8. 3 message was significantly greater in the eyes of C. AL-20 mice; Vβ8. 1 was expressed only in CB-17 mice. There were obvious differences in the TCR Vβ expression between HSK-susceptible and HSK-resistant mice. The differences in the intensity of the message expressed by Vβ8 family members between the two strains could be correlated to previous experiments that showed Vβ8. 1, 2+ T cells as the main infiltrating cells in the corneas of HSK-susceptible mice by day 11 and 14 after challenge with HSV-1.