Molecular responses to aphid feeding in Arabidopsis in relation to plant defense pathways
Molecular responses to aphid feeding in Arabidopsis in relation to plant defense pathways
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DOI：
10.1104/pp.125.2.1074
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发表时间：
2001-02-01
期刊：
影响因子：
7.4
通讯作者：
Thompson, GA
中科院分区：
文献类型：
--
作者：
Moran, PJ;Thompson, GA
关于植物对昆虫取食韧皮部的分子反应知之甚少。在绿桃蚜（Myzus persicae）以拟南芥为食后，研究了与伤口和病原体反应途径相关的基因的诱导。以莲座叶为食的蚜虫诱导与水杨酸 (SA) 依赖性病原体反应相关的两个基因 (PR-1 和 BGL2) 的转录分别提高了 10 倍和 23 倍。在 SA 信号传导缺陷的 npr1 突变植物中，PR-1 和 BGL2 mRNA 的诱导减少。应用SA类似物苯并噻二唑导致野生型植物和缺乏SA反应性的突变植物叶子上的蚜虫繁殖减少，表明野生型SA依赖性反应不会影响对蚜虫的抗性。 PDF1.2 的 mRNA 水平增加了两倍，PDF1.2 编码防御素，一种参与茉莉酸 (JA)/乙烯依赖性反应途径的肽。编码 JA 诱导型脂氧合酶 (LOX2) 和 SA/JA 诱导型 Phe-氨裂解酶的转录本增加了 1.5 至 2 倍。在JA抗性coil-1突变体的受侵染叶片中，蚜虫对PDF1.2和LOX2的诱导并未发生。蚜虫取食后，与应激相关的单糖同向转运蛋白 STP4 的 mRNA 水平增加了 10 倍。以拟南芥为食的韧皮部会刺激与病原体感染和受伤相关的反应途径。
Little is known about molecular responses in plants to phloem feeding by insects. The induction of genes associated with wound and pathogen response pathways was investigated following green peach aphid (Myzus persicae) feeding on Arabidopsis. Aphid feeding on rosette leaves induced transcription of two genes associated with salicylic acid (SA)dependent responses to pathogens (PR-1 and BGL2) 10- and 23-fold, respectively. Induction of PR-1 and BGL2 mRNA was reduced in npr1 mutant plants, which are deficient in SA signaling. Application of the SA analog benzothiadiazole led to decreases in aphid reproduction on leaves of both wild-type plants and mutant plants deficient in responsiveness to SA, suggesting that wild-type SA-dependent responses do not influence resistance to aphids. Two-fold increases occurred in mRNA levels of PDF1.2, which encodes defensin, a peptide involved in the jasmonate (JA)- / ethylene-dependent response pathway. Transcripts encoding JA-inducible lipoxygenase (LOX2) and SA/JA-inducible Phe-ammonia lyase increased 1.5-to 2-fold. PDF1.2 and LOX2 induction by aphids did not occur in infested leaves of the JA-resistant coil-1 mutant. Aphid feeding induced 10-fold increases in mRNA levels of a stress-related monosaccharide symporter gene, STP4. Phloem feeding on Arabidopsis leads to stimulation of response pathways associated with both pathogen infection and wounding.