Regulation of phenazine-1-carboxamide production by quorum sensing in type strains of Pseudomonas chlororaphis subsp. chlororaphis and Pseudomonas chlororaphis subsp. piscium
Regulation of phenazine-1-carboxamide production by quorum sensing in type strains of Pseudomonas chlororaphis subsp. chlororaphis and Pseudomonas chlororaphis subsp. piscium
复制标题
DOI：
10.1016/j.jbiosc.2022.03.004
复制
发表时间：
2022-06-09
影响因子：
2.8
通讯作者：
Someya, Nobutaka
中科院分区：
文献类型：
--
作者：
Morohoshi, Tomohiro;Yabe, Naoka;Someya, Nobutaka
群体感应是一种群体密度依赖的基因调控机制。N-酰基-L-高丝氨酸内酯（阿勒）是革兰氏阴性菌群体感应的信号物质。吩嗪类化合物是一类具有广谱抗真菌活性的细菌次级代谢产物。绿针假单胞菌是一种生防菌株，其大多数种类都能通过AHL介导的群体感应产生吩嗪类化合物。虽然绿针假单胞菌被分为四个亚种，但在其中两个亚种中，绿针假单胞菌亚种（P. chlororaphis subsp.）chlororaphis和piscium。在绿针假单胞菌（P. chlororaphis subsp.）绿针霉（P. chlororaphis）JCM 2778（T）和绿针霉（P. chlororaphis subsp.）piscium DSM 21509（T）.在JCM 2778和DSM 21509样品中检测到两种主要AHL，N-（3-羟基己酰基）-L-高丝氨酸内酯和N-（3-羟基辛酰基）-L-高丝氨酸内酯。PhzI合成所有AHL;然而，CsaI不能在JCM 2778和DSM 21509中进行阿勒生物合成。在这两种菌株中，破坏phzI导致吩嗪-1-羧酸（PCA）和吩嗪-1-甲酰胺（PCN）的生产完全消失，但是，破坏CsaI没有引起显着的变化，PCA和PCN生产。JCM 2778和DSM 21509在群体感应下产生的吩嗪衍生物对于控制植物病原真菌立枯丝核菌、禾谷镰刀菌和尼伦伯格镰刀菌是至关重要的。这些结果表明，PhzI/PhzR群体感应系统在吩嗪衍生物的合成和生防活性方面发挥着重要作用。(C)2022年，生物技术学会，日本。All rights reserved.
Quorum sensing is a population density-dependent gene regulation mechanism. N-Acyl-L-homoserine lactone (AHL) has been identified as a signal compound in quorum sensing in gram-negative bacteria. Phenazine derivatives are bacterial secondary metabolites known for their broad-spectrum antifungal activity. Pseudomonas chlororaphis has been demonstrated to be a biocontrol strain, and most of its species can produce phenazine derivatives under AHL-mediated quorum sensing. Although P. chlororaphis is divided into four subspecies, the relationship between phenazine production and quorum sensing has not been investigated in two of the subspecies, P. chlororaphis subsp. chlororaphis and piscium. Two luxI/luxR homolog gene sets, phzI and phzR and csaI and csaR, were found in the complete genome sequences of the type strains of P. chlororaphis subsp. chlororaphis JCM 2778(T) and P. chlororaphis subsp. piscium DSM 21509(T). Two major AHLs, N-(3-hydroxyhexanoyl)-L-homoserine lactone and N-(3-hydroxyoctanoyl)-L-homoserine lactone, were detected in JCM 2778 and DSM 21509 samples. PhzI synthesized all AHLs; however, CsaI could not perform AHL biosynthesis in JCM 2778 and DSM 21509. In both strains, disruption of the phzI caused complete disappearance of phenazine-1-carboxylic acid (PCA) and phenazine-1-carboxamide (PCN) production; however, disruption of csaI did not induce significant changes in PCA and PCN production. Phenazine derivatives produced by JCM 2778 and DSM 21509 under quorum sensing are crucial for the control of the plant pathogenic fungi, Rhizoctonia solani, Fusarium graminearum, and Fusarium nirenbergiae. These results demonstrated that PhzI/PhzR quorum-sensing system play an important role in production of phenazine derivatives and biocontrol activity. (C) 2022, The Society for Biotechnology, Japan. All rights reserved.