Generation of unique mono-hydroxy-eicosatetraenoic acids from arachidonic acid by human neutrophils.
Generation of unique mono-hydroxy-eicosatetraenoic acids from arachidonic acid by human neutrophils.
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DOI：
10.1084/jem.150.2.406
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发表时间：
1979-08-01
影响因子：
15.3
通讯作者：
Sun, F F
中科院分区：
文献类型：
--
作者：
Goetzl, E J;Sun, F F
在存在吲哚美辛的情况下，将[3 H]花生四烯酸与来自人中性粒细胞匀浆的17，000-g上清液孵育，除12-HETE、11-HETE和5-HETE外，还产生独特的代谢物9-OH-5，7，11，14-二十碳四烯酸（9-HETE）和8-HETE。HETE产品的人中性粒细胞趋化活性表现出效力的等级顺序，5-HETE大于8-HETE = 9-HETE大于11-HETE = 12-HETE。表达的趋化动力学活性以及趋化活性表明，这些原则的内源性生产可能会影响人类中性粒细胞的流动性。
Incubation of [3H]arachidonic acid with the 17,000-g supernatant from homogenates of human neutrophils in the presence of indomethacin generated the unique metabolites 9-OH-5,7,11,14-eicosatetraenoic acid (9-HETE) and 8-HETE, in addition to 12-HETE, 11-HETE and 5-HETE. The human neutrophil chemotactic activity of the HETE products exhibited a rank-order of potency with 5-HETE greater than 8-HETE = 9-HETE greater than 11-HETE = 12-HETE. The expression of chemokinetic activity as well as chemotactic activity suggested that the endogenous production of these principles may influence the mobility of human neutrophils.