Multiple forms of IFN-beta 2/IL-6 in serum and body fluids during acute bacterial infection.
Multiple forms of IFN-beta 2/IL-6 in serum and body fluids during acute bacterial infection.
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DOI：
10.4049/jimmunol.142.3.948
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发表时间：
1989-02
影响因子：
4.4
通讯作者：
D. Helfgott;S. Tatter;U. Santhanam;R. H. Clarick;N. Bhardwaj;L. May;P. Sehgal
中科院分区：
文献类型：
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作者：
D. Helfgott;S. Tatter;U. Santhanam;R. H. Clarick;N. Bhardwaj;L. May;P. Sehgal
肝脏急性期反应所特有的血浆蛋白的许多主要变化由干扰素-β2/IL-6调节。应用干扰素-β2/IL-6的特异性生物测定，我们检测了急性细菌感染患者体液中高水平的干扰素-β2/IL-6。该方法依赖于人肝癌细胞株Hep3B克隆体诱导肝脏急性时相血浆蛋白α1-抗糜蛋白酶，并用抗rIFN-β2/IL-6抗血清抑制其活性。4例急性细菌性脑膜炎患者(肺炎链球菌、金黄色葡萄球菌、2例单核细胞增多性李斯特菌)脑脊液中均有较高水平的干扰素-β2/白介素6(高达500 ng/ml)。其中2例合并菌血症患者血清中的干扰素-β2/IL-6浓度较低(5~70 ng/ml)。另外三名患有大肠埃希菌、铜绿假单胞菌和脑膜炎奈瑟菌菌血症的患者血清中的干扰素-β2/白介素6水平较高，1名犬链球菌关节炎患者的踝液中也有类似的水平。正常脑脊液和血清中几乎检测不到干扰素-β2/IL-6。采用免疫亲和层析和免疫印迹相结合的方法，对具有代表性的血清和脑脊液样本中存在的干扰素-β2/白介素6进行了鉴定。在人体体液中检测到23~30 kDa的多种干扰素-β2/IL-6免疫活性物质和60~70 kDa的免疫活性复合体。这是第一次证明之前对细胞培养中产生的人干扰素-β2/IL-6物种的异质性的描述与在受感染宿主中观察到的一致。
Many of the major alterations in plasma proteins characteristic of the hepatic acute phase response are regulated by IFN-beta 2/IL-6. Using a specific bioassay for IFN-beta 2/IL-6, which relies on the induction of the hepatic acute phase plasma protein alpha 1-antichymotrypsin in the human hepatoma cell line Hep3B clone 2 and its inhibition by anti-rIFN-beta 2/IL-6 antiserum, we have detected high levels of IFN-beta 2/IL-6 in the body fluids of patients with acute bacterial infections. Cerebrospinal fluid from four patients with acute bacterial meningitis (Streptococcus pneumoniae, Staphylococcus aureus, two cases of Listeria monocytogenes) all had high levels of IFN-beta 2/IL-6 (up to 500 ng/ml). Two of these patients with concomitant bacteremia had lower concentrations of IFN-beta 2/IL-6 in the serum (5 to 70 ng/ml). Three additional patients with Escherichia coli, Pseudomonas aeruginosa, and Neisseria meningitidis bacteremia had high levels of serum IFN-beta 2/IL-6, as did the ankle fluid of a patient with Streptococcus canis arthritis. Normal cerebrospinal fluid and serum had little detectable IFN-beta 2/IL-6. A combination of immunoaffinity chromatography and immunoblotting procedures were used to characterize the IFN-beta 2/IL-6 species present in a representative sampling of serum and cerebrospinal fluids. Multiple immunoreactive species of IFN-beta 2/IL-6 in the size range 23 to 30 kDa as well as immunoreactive complexes in the range 60 to 70 kDa were detected in human body fluids. This is the first demonstration that previous descriptions of heterogeneity in human IFN-beta 2/IL-6 species produced in cell culture correspond to observations in the infected host.