Cloning of full-length zebrafish dcc and expression analysis during embryonic and early larval development
Cloning of full-length zebrafish dcc and expression analysis during embryonic and early larval development
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DOI：
10.1002/dvdy.20492
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发表时间：
2005-11-01
影响因子：
2.5
通讯作者：
Chien, CB
中科院分区：
文献类型：
--
作者：
Fricke, C;Chien, CB
DCC 家族成员在脊椎动物和无脊椎动物的轴突引导中发挥着关键作用。在斑马鱼中，迄今为止仅报道了 dcc 基因的一小部分序列。在这里，我们报道了全长斑马鱼 DCC 的克隆。斑马鱼 DCC 具有免疫球蛋白超家族 DCC 亚组的典型结构，由胞外结构域中的 4 个免疫球蛋白和 6 个纤连蛋白 III 型重复序列、单个跨膜结构域和具有 3 个保守基序的胞内结构域组成。作为研究 dcc 功能的第一步，我们分析了其序列并表征了其在胚胎和幼虫发育过程中的表达模式。 dcc 在脑和脊髓中高表达，但在发育中的胸鳍、胰腺、肠和心脏中也观察到明显的染色。因此，dcc 可能不仅在轴突引导中发挥作用，而且还在这些器官的形态发生和功能中发挥作用。
Members of the DCC family play key roles in axon guidance in vertebrates as well as invertebrates. In zebrafish, only a short partial sequence of the dcc gene has been reported to date. Here, we report the cloning of full-length zebrafish dcc. Zebrafish DCC shares the typical structure of the DCC subgroup of the immunoglobulin superfamily, consisting of four immunoglobulin and six fibronectin-type III repeats in the extracellular domain, a single transmembrane domain, and an intracellular domain with three conserved motifs. As a first step toward studying the function of dcc, we analyzed its sequence and characterized its expression pattern during embryonic and larval development. dcc is expressed highly in brain and spinal cord, but distinct staining was also observed in developing pectoral fins, pancreas, intestine, and heart. Thus, dcc may play roles not only in axon guidance, but in morphogenesis and functioning of these organs as well.