Protective effects of rutin on lipopolysaccharide-induced heart injury in mice
Protective effects of rutin on lipopolysaccharide-induced heart injury in mice
复制标题
芦丁对脂多糖所致小鼠心脏损伤的保护作用
DOI：
10.2131/jts.43.329
复制
发表时间：
2018-01-01
影响因子：
2
通讯作者：
Mo Yanzhi
中科院分区：
文献类型：
--
作者：
Liu Xianchu;Zheng Lan;Mo Yanzhi
由于其多种生物学效应，大黄在改善多器官损伤方面具有广泛的有益健康特性。本研究旨在观察芦丁对脂多糖（LPS）诱导的心肌损伤的影响，并探讨其潜在的心肌保护机制。采用LPS腹腔感染小鼠建立心脏损伤模型，并连续8 d灌胃给予芦丁。注射LPS后1d，检测心脏组织病理学、心肌标志酶及心肌纤维化相关基因的表达，评价芦丁的心肌保护作用。此外，还检测了氧化指标和炎性细胞因子，以探讨其可能的潜在机制。结果表明，芦丁能明显改善LPS诱导的脓毒症大鼠心肌形态学改变，降低心肌标志酶肌酸激酶（CK）和乳酸脱氢酶（LDH）的水平，对心脏有保护作用。此外，芦丁还能显著降低心肌纤维化相关基因[基质金属蛋白酶2（MMP-2）和基质金属蛋白酶9（MMP-9）]的表达，从而预防LPS诱导的心肌纤维化。此外，芦丁显着增加抗氧化酶[超氧化物歧化酶（SOD）和过氧化氢酶（CAT）]的活性，并提高氧化产物[丙二醛（MDA）和H2 O2]水平，以平衡氧化和抗氧化系统在心脏。最后，芦丁显著改善了[肿瘤坏死因子α（TNF-α）和白细胞介素6（IL-6）]的活性，以抑制心脏中的炎症反应。结论：芦丁具有抗氧化和抗炎作用，可改善LPS诱导的心肌损伤，提示芦丁可作为脓毒症心肌保护药物。
Rutin has a wide range of beneficial health properties in the amelioration of multi-organ injury owing to its various biological effects. The aim of this study was to investigate the effects of rutin on lipopolysaccharide (LPS)-induced heart injury and clarify its potential cardioprotective mechanism. The mouse model of heart injury was intraperitoneal infection with LPS, and rutin was orally administered for 8 consecutive days. One day after LPS injection, heart histopathology, cardiac marker enzymes and cardiac fibrosis related genes were determined to evaluate the cardioprotective effects of rutin. In addition, oxidative parameters and inflammatory cytokines were tested to explore its possible underlying mechanism. The presented results showed that rutin significantly improved morphological changes of myocardium and relieved cardiac marker enzymes [creatine kinase (CK) and lactate dehydrogenase (LDH)] level to protect heart in LPS-induced sepsis. And more, rutin observably mitigated fibrosis related genes [matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9)] expression in the heart to prevent against LPS-induced cardiac fibrosis. In addition, rutin markedly increased antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)] activity, and improved oxidative production [malondialdehyde (MDA) and H2O2] level to balance the oxidation and anti-oxidation systems in the heart. Lastly, rutin dramatically ameliorated [tumor necrosis factor alpha (TNF-alpha) and interleukin 6 (IL-6)] activity to restrain inflammatory responses in the heart. In conclusion, rutin possessed anti-oxidant and anti-inflammatory properties to improve LPS-induced heart injury, which suggested rutin could be used as a potential cardioprotective medicine in sepsis.