Drosophila ptip is essential for anterior/posterior patterning in development and interacts with the PcG and trxG pathways
Drosophila ptip is essential for anterior/posterior patterning in development and interacts with the PcG and trxG pathways
复制标题
果蝇 ptip 对于发育过程中的前/后模式至关重要，并与 PcG 和 trxG 通路相互作用
DOI：
10.1242/dev.026559
复制
发表时间：
2009-06-01
期刊：
影响因子：
4.6
通讯作者：
Dressler, Gregory R.
中科院分区：
文献类型：
--
作者：
Fang, Ming;Ren, Hongyan;Dressler, Gregory R.
果蝇的发育在一定程度上依赖于多梳和三胸蛋白质组的协同作用进行的表观遗传调节，其中许多蛋白质与组蛋白甲基转移酶活性有关。小鼠PTIP是组蛋白H3K4甲基转移酶复合体的一部分，含有6个BRCT结构域和一个富含谷氨酰胺的区域。在这篇文章中，我们描述了哺乳动物PTIP(Paxip1)基因的果蝇同源基因在早期发育和想象中的椎间盘模式中的重要作用。在幼虫发育过程中，母体和受精卵的PTIP都是分割和轴线模式所必需的。PTIP的缺失导致全球H3K4甲基化水平的下降和H3K27甲基化水平的上升。在细胞培养中，果蝇PTIP需要激活同源基因的表达，以响应Polycomb组基因的去抑制。发育基因的激活与PTIP蛋白与启动子序列的结合和H3K4三甲基化的增加是一致的。这些数据表明，PTIP在发育和分化的表观遗传控制中具有高度保守的功能。
Development of the fruit fly Drosophila depends in part on epigenetic regulation carried out by the concerted actions of the Polycomb and Trithorax group of proteins, many of which are associated with histone methyltransferase activity. Mouse PTIP is part of a histone H3K4 methyltransferase complex and contains six BRCT domains and a glutamine-rich region. In this article, we describe an essential role for the Drosophila ortholog of the mammalian Ptip (Paxip1) gene in early development and imaginal disc patterning. Both maternal and zygotic ptip are required for segmentation and axis patterning during larval development. Loss of ptip results in a decrease in global levels of H3K4 methylation and an increase in the levels of H3K27 methylation. In cell culture, Drosophila ptip is required to activate homeotic gene expression in response to the derepression of Polycomb group genes. Activation of developmental genes is coincident with PTIP protein binding to promoter sequences and increased H3K4 trimethylation. These data suggest a highly conserved function for ptip in epigenetic control of development and differentiation.