Trehalose protects Saccharomyces cerevisiae from lipid peroxidation. during oxidative stress
Trehalose protects Saccharomyces cerevisiae from lipid peroxidation. during oxidative stress
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DOI：
10.1016/j.bbagen.2006.01.010
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发表时间：
2006-03-01
影响因子：
3
通讯作者：
Eleutherio, ECA
中科院分区：
文献类型：
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作者：
Herdeiro, RS;Pereira, MD;Eleutherio, ECA
为了研究海藻糖在氧化条件下的保护作用，采用了两组具有不同海藻糖合成谱的酿酒酵母菌株。细胞用10%海藻糖溶液或热处理（导致海藻糖积累）处理，然后暴露于甲萘醌（超氧化物的来源）或叔丁基过氧化氢（TBOOH）。根据我们的研究结果，海藻糖在暴露于甲萘醌胁迫下显着增加了生存能力，这似乎与脂质过氧化水平的降低有关。在SOD缺乏的情况下，海藻糖对甲萘醌氧化损伤的保护作用特别有效。另一方面，由于海藻糖预处理（添加外部海藻糖）不能提高细胞存活率，这种糖似乎不参与对TBOOH耐受性的获得机制。因此，海藻糖起着保护细胞，尤其是细胞膜免受氧化损伤的作用。然而，这种防御机制取决于细胞所面临的氧化应激类型。(C) 2006 Elsevier B.V.版权所有
Aiming to focus the protective role of the sugar trehalose under oxidative conditions, two sets of Saccharomyces cerevisiae strains, having different profiles of trehalose synthesis, were used. Cells were treated either with a 10% trehalose solution or with a heat treatment (which leads to trehalose accumulation) and then exposed either to menadione (a source of superoxide) or to tert-butylhydroperoxide (TBOOH). According to our results, trehalose markedly increased viability upon exposure to menadione stress, which seems to be correlated with decrease in lipid peroxidation levels. The protective effect of trehalose against oxidative damage produced by menadione was especially efficient under SOD] deficiency. On the other hand, this sugar does not seem to participate of the mechanism of acquisition of tolerance against TBOOH, since trehalose pretreatment (addition of external trehalose) was not capable of increase cell survival. Therefore, trehalose plays a role in protecting cells, especially membranes, from oxidative injuries. However, this mechanism of defense is dependent on the type of oxidative stress to which cells are submitted. (C) 2006 Elsevier B.V. All rights reserved.