Stromal Cell-Derived Factor-1 Enhances Wound Healing through Recruiting Bone Marrow-Derived Mesenchymal Stem Cells to the Wound Area and Promoting Neovascularization
Stromal Cell-Derived Factor-1 Enhances Wound Healing through Recruiting Bone Marrow-Derived Mesenchymal Stem Cells to the Wound Area and Promoting Neovascularization
复制标题
基质细胞衍生因子 1 通过将骨髓衍生间充质干细胞募集到伤口区域并促进新血管形成来增强伤口愈合
DOI：
10.1159/000342921
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发表时间：
2013-01-01
影响因子：
2.7
通讯作者：
Huang, Hong
中科院分区：
文献类型：
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作者：
Xu, Xiang;Zhu, Fangqiang;Huang, Hong
基质细胞衍生因子-1 （SDF-1）是一种有效的趋化因子，用于表达SDF-1受体CXCR4的骨髓源性基质干细胞（BMSCs）。SDF-1被认为在骨髓间充质干细胞的贩运中起重要作用。我们研究了SDF-1对BMSCs在创面招募的贡献，以及它对伤口修复和新生血管的促进作用。分别用或不加抗cxcr4阻断抗体预处理骨髓间充质干细胞并联合CM-DiI标记，经尾静脉注射到背部全层皮肤创面小鼠体内。同时，另一组小鼠局部创面注射抗sdf -1抗体。结果表明，CXCR4阻断宿主伤口中输注的骨髓间充质干细胞或SDF-1(1)显著降低了输注的骨髓间充质干细胞向损伤组织募集的数量；(2)降低了参与损伤组织修复的生长因子，如血管内皮生长因子、碱性成纤维细胞生长因子和转化生长因子β 1的表达；(3)减少了由此产生的新生血管；(4)延缓了伤口愈合。综上所述，研究结果表明，SDF-1/CXCR4信号通路通过增加BMSCs向伤口组织的募集、BMSCs分泌生长因子和伤口区域的新生血管来促进伤口愈合。
Stromal cell-derived factor-1 (SDF-1) is a potent chemokine for bone marrow-derived stromal stem cells (BMSCs) that express CXCR4, the receptor for SDF-1. SDF-1 is considered to play an important role in the trafficking of BMSCs. We investigated the contribution of SDF-1 to the recruitment of BMSCs to the wound area and its promotion of wound repair and neovascularization. BMSCs were pretreated with or without anti-CXCR4 blocking antibody and combined with CM-DiI label, and injected via the tail vein into mice with full-thickness skin wounds on the dorsum. Simultaneously, anti-SDF-1 antibody was injected into local wounds in another group of mice. The results show that blockade of CXCR4 on either infused BMSCs or SDF-1 in the host wounds (1) dramatically impaired the number of infused BMSCs being recruited to the injured tissue, (2) reduced the expression of growth factors involved in the repair of injured tissue such as vascular endothelial growth factor, basic fibroblast growth factor and transforming growth factor beta 1, (3) decreased the resultant neovascularization, and (4) retarded wound healing. Taken together, the findings indicate that the SDF-1/CXCR4 signal pathway facilitates wound healing through augmenting BMSC recruitment to wound tissues, responsive secretion of growth factors by BMSCs and neovascularization in the wound area.