ASSEMBLY OF YEAST SEC PROTEINS INVOLVED IN TRANSLOCATION INTO THE ENDOPLASMIC-RETICULUM INTO A MEMBRANE-BOUND MULTISUBUNIT COMPLEX
ASSEMBLY OF YEAST SEC PROTEINS INVOLVED IN TRANSLOCATION INTO THE ENDOPLASMIC-RETICULUM INTO A MEMBRANE-BOUND MULTISUBUNIT COMPLEX
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DOI：
10.1038/349806a0
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发表时间：
1991-02-28
期刊：
影响因子：
64.8
通讯作者：
SCHEKMAN, R
中科院分区：
文献类型：
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作者：
DESHAIES, RJ;SANDERS, SL;SCHEKMAN, R
分泌蛋白转运到内质网（ER）被认为是由整体膜蛋白催化。遗传选择揭示了三个酿酒酵母基因（SEC61， SEC62和SEC63），这些突变在体内1-3和体外2-4阻断了前体蛋白进入内质网腔的输入。SEC62（参考文献4）和SEC63（参考文献5）的DNA序列预测了多跨膜蛋白，并且SEC62蛋白（SEC62）的生化表征证实了它是一个完整的ER膜蛋白6。本研究表明Sec61、Sec62和Sec63与另外两种蛋白一起组装成一个多亚基膜相关复合物。这些结果证实了先前的预测，基于SEC基因之间的遗传相互作用，即Sec61、Sec62和Sec63共同作用，促进蛋白质转运到内质网。
SECRETORY-protein translocation into the endoplasmic reticulum (ER) is thought to be catalysed by integral membrane proteins. Genetic selections uncovered three Saccharomyces cerevisiae genes (SEC61, SEC62 and SEC63), mutations in which block import of precursor proteins into the ER lumen in vivo 1-3 and in vitro 2-4. The DNA sequences of SEC62 (ref. 4) and SEC63 (ref. 5) predict multispanning membrane proteins, and biochemical characterization of the SEC62 protein (Sec62) confirms that it is an integral ER membrane protein6. Here we show that Sec61, Sec62 and Sec63 are assembled with two additional proteins into a multisubunit membrane-associated complex. These results confirm previous predictions, based upon genetic interactions between the SEC genes, that Sec61, Sec62 and Sec63 act together to facilitate protein translocation into the ER.