Visual observation of African trypanosomes during cryopreservation.
Visual observation of African trypanosomes during cryopreservation.
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DOI：
10.1089/bio.2014.0011
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发表时间：
2014-08
影响因子：
1.6
通讯作者：
Hai-Yan Wang;Y. Wen;Z. Lun;Shu-shen Lu
中科院分区：
文献类型：
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作者：
Hai-Yan Wang;Y. Wen;Z. Lun;Shu-shen Lu
原生动物已被广泛用于超低温保存的研究。冻存后的存活率一直是人们关注的焦点，而冻融过程中细胞活力的研究较少。在本研究中，我们报告了成功的冻存布氏锥虫，一种人类和动物的寄生原生动物，使用控制速率冷冻在5°C/min，并使用显微镜差示扫描量热仪系统在冷冻和解冻过程中的活性实时观察。用0.4M海藻糖作为冷冻保护剂，可使锥虫耐受冻融，存活率>89%。机制分析结果表明，海藻糖玻璃化冷冻对低温下锥虫的损伤有明显的保护作用。
Protozoa have been widely used for the study of cryopreservation. The survival rate after cryopreservation has always received the most attention, while the cell viability during the process of freezing and thawing has been much less studied. In the present study, we report successful cryopreservation of Trypanosoma brucei, a parasitic protozoa of human and animals, using controlled-rate freezing at 5°C/min, and real-time observation of activity using a microscope differential scanning calorimeter system during the freezing and thawing process. Trehalose used as a cryoprotective agent at a concentration of 0.4 M allowed the trypanosomes to endure freezing and thawing with >89% survival rate. Results from mechanisms analysis indicate that vitrification by trehalose contributes significantly to the protection of the trypanosomes from damage at low temperature.