Expression of slit-2 and slit-3 during chick development
Expression of slit-2 and slit-3 during chick development
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DOI：
10.1002/dvdy.1182
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发表时间：
2001-10-01
影响因子：
2.5
通讯作者：
Niswander, L
中科院分区：
文献类型：
--
作者：
Holmes, G;Niswander, L
果蝇和脊椎动物的狭缝蛋白已被表征为被机器人受体蛋白识别的分泌的化学驱避剂，其主要功能是引导神经元轴突和神经元。狭缝基因也在肢体中表达。为了给肢体中狭缝功能的测定提供基础，我们分离并表征了鸡胚胎发育中的肢体和其他组织中鸡狭缝2和狭缝3的表达。与哺乳动物同源基因相比，这两种基因在鸡胚中具有相似的表达谱，特别是在神经管中。在肢体中，它们的表达模式表明它们参与肢体发育的许多方面。在早期肢芽中，slit-2在外周间质和侵入的肌肉前体中表达，而slit-3表达仅限于肢芽未来的软骨形成核心。在后期阶段，两个缝隙基因均在指间间充质、内部骨膜细胞以及紧邻骨膜的放射状和表皮下的间充质中表达。 lit-3也在软骨发育过程中的增殖软骨细胞中表达，而slit-2则在后期的肌肉块和自足关节的外周表达。 (C) 2001 Wiley-Liss, Inc.
The Drosophila and vertebrate slit proteins have been characterized as secreted chemorepellents recognized by the robo receptor proteins that function principally for the guidance of neuronal axons and neurons. slit genes are also expressed in the limb. To provide a basis for the determination of slit functions in the limb we have isolated and characterized the expression of chick slit-2 and slit-3 in the developing limb and other tissues of the chick embryo. Both genes share similar expression profiles in the chick embryo when compared to that of their mammalian homologues, particularly in the neural tube. In the limb, their expression patterns suggest their involvement in many aspects of limb development. In the early limb bud, slit-2 is expressed in the peripheral mesenchyme and invading muscle precursors, while slit-3 expression is restricted to the future chondrogenic core of the limb bud. At later stages, both slit genes are expressed in interdigital mesenchyme, in inner periosteal cells, and in mesenchyme immediately radial to the periosteum and under the epidermis. slit-3 is also expressed in proliferating chondrocytes during cartilage development, while slit-2 is expressed in later muscle masses and peripherally to joints in the autopod. (C) 2001 Wiley-Liss, Inc.