Microscopic method for assaying F cell production: illustrative changes during infancy and in aplastic anemia.
Microscopic method for assaying F cell production: illustrative changes during infancy and in aplastic anemia.
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测定 F 细胞产生的显微方法：婴儿期和再生障碍性贫血期间的说明性变化。
作者：
G. Dover;Samuel H. Boyer;William R. Bell
通过对显微镜单细胞径向免疫扩散法的改进，可以在外周血中检测到携带胎儿血红蛋白（HbF）的网状细胞（F网状细胞）。因此，可以很容易地识别出HbF产生的其他不可察觉的变化。当全血含hbf红细胞（F细胞）频率和网织红细胞频率的乘积约为5 × 10（-4）或更大时，网织红细胞频率就有报告可测。对F网织细胞和非网织细胞F细胞（F红细胞）的一系列分析表明：(1)F网织细胞和F红细胞的水平在正常成人中持续相似，F细胞超过6%，因此F细胞和非F细胞的存活时间本质上是相同的；(2)F网织细胞水平的变化可以敏感地预测婴儿期和再生障碍性贫血恢复期成熟F细胞频率的变化。(3)网织细胞频率和每F网织细胞HbF量的变化在某些情况下不一致，但在其他情况下一致。
Fetal hemoglobin (HbF)-bearing reticulocytes (F reticulocytes) can be detected in peripheral blood by a modification of the microscopic single-cell radial immunodiffusion method. Thereby otherwise inappreciable changes in HbF production can readily be recognized. F reticulocyte frequencies are reporducibly measurable whenever the product of whole blood HbF-bearing red cell (F cell) frequency and reticulocyte frequency is approximately 5 X 10(-4) or greater. Serial analyses of F reticulocytes and nonreticulocyte F cells (F erythrocytes) illustrate that (1) levels of F reticulocytes and F erythrocytes are persistently similar in normal adults with more than 6% F cells and thus cell survival times of F and non-F cells must be essentially the same, (2) changing levels of F reticulocytes can be sensitive predictors of later changes in mature F cell frequencies during infancy and in adults recovering from aplastic anemia, and (3) alterations in F reticulocyte frequency and the amount of HbF per F reticulocyte are discordant in some settings but concordant in others.