Maintaining and Monitoring the Defined Microbiota Status of Gnotobiotic Rodents
Maintaining and Monitoring the Defined Microbiota Status of Gnotobiotic Rodents
复制标题
DOI：
10.1093/ilar/ilv029
复制
发表时间：
2015-01-01
期刊：
影响因子：
2.5
通讯作者：
Bleich, Andre
中科院分区：
文献类型：
--
作者：
Nicklas, Werner;Keubler, Lydia;Bleich, Andre
gnotobiotic（无潮，定义的殖民化）啮齿动物已成为提高我们对宿主微生物关系关系的理解的强大工具。然而，在无毛动物中必须证明，维持且最终监测gnotobiotic啮齿动物是一个关键，劳动密集型且昂贵的过程（例如，不育，不存在特定病原体）。在这里，我们提供有关gnotobiotic动物的住房和维护，阐明预防性测量以避免污染的信息，并为采样程序，采样频率和测试方法提出具体建议。
Gnotobiotic (germfree, defined colonized) rodents have become powerful tools to advance our understanding of the host-microbiome relationship. However, the maintenance and ultimately the monitoring of gnotobiotic rodents is a critical, labor-intensive, and costly process (e.g., sterility, not absence of specific pathogens, must be demonstrated in germfree animals). Here, we provide information on the housing and maintenance of gnotobiotic animals, elucidate prophylactic measurements to avoid contamination, and make specific recommendations for sampling procedures, sampling frequencies, and test methods.