Pollen Tube Growth, Fertilization and Seed Development in Hyuganatsu (Citrus tamurana) Seedless Cultivars 'Muroto-Konatsu' and 'Nishiuchi-Konatsu'
Pollen Tube Growth, Fertilization and Seed Development in Hyuganatsu (Citrus tamurana) Seedless Cultivars 'Muroto-Konatsu' and 'Nishiuchi-Konatsu'
复制标题
Hyuganatsu（柑橘）无核品种“Muroto-Konatsu”和“Nishiuchi-Konatsu”的花粉管生长、受精和种子发育
DOI：
10.2503/jjshs.70.320
复制
发表时间：
2001
影响因子：
--
通讯作者：
K. Hasegawa
中科院分区：
文献类型：
--
作者：
A. Kitajima;Yoshikiyo Okada;K. Hasegawa
“Muroto-Konatsu”和“Nishiuchi-Konatsu”是Hyuganatsu（柑橘）的无籽芽突变体，研究了它们的无籽基础。1. 在子房胚珠数和子房室数上，“室多- konatsu”、“西内- konatsu”和普通型Hyuganatsu没有显著差异。当‘Muroto-Konatsu’、‘nishichi - konatsu’和普通型用‘sui - buntan’的花粉授粉时，花粉管几乎没有穿透‘Muroto-Konatsu’的雌蕊，而‘nishichi - konatsu’和普通型的花粉管则向下生长到子房。因此，“Muroto-Konatsu”有少数受精的胚珠未能结实，而“Nishiuchi-Konatsu”和普通类型在授粉后14天的子房上部有相同数量的花粉管，并且受精胚珠和种子的数量相等。3. “西野- konatsu”和普通型进行了自花授粉和互花授粉。在自花授粉的‘Nishiuchi-Konatsu’中，花粉管穿透到子房的上部。因此，产生了一些受精的胚珠。在互花授粉的‘Nishiuchi-Konatsu’和自花授粉的‘普通型’中，花粉管只穿透花柱的中部。因此没有受精的胚珠产生。这些数据表明，‘Muroto-Konatsu’的无籽性是由于异花授粉时花粉管生长受到抑制，而在‘Nishiuchi-Konatsu’中，由于花粉生长不良，自花授粉时几乎总是无籽的。结果表明，无籽栽培品种“Muroto-Konatsu”和“Nishiuchi-Konatsu”的雌蕊性状在杂交亲和性和自交亲和性上分别发生了突变。
'Muroto-Konatsu' and 'Nishiuchi-Konatsu' are seedless bud mutants of Hyuganatsu (Citrus tamurana) which were examined for their bases of seedlessness. 1. There were no significant differences in the numbers of ovules and locules per ovary among 'Muroto-Konatsu', 'Nishiuchi-Konatsu' and common type Hyuganatsu. 2. When 'Muroto-Konatsu', 'Nishiuchi-Konatsu' and the common type were pollinated with pollen from 'Suisho-Buntan' pummelo, the pollen tubes barely penetrated the pistil of 'Muroto-Konatsu', whereas they grew down to the ovary of 'Nishiuchi-Konatsu' and the common type. Hence, 'Muroto-Konatsu' had a few fertilized ovules which failed to set seeds, whereas 'Nishiuchi-Konatsu' and the common type had the same number of pollen tubes in the upper part of their ovaries 14 day after pollination and set an equal of fertilized ovules and seeds. 3. Self-pollination and reciprocal pollination were performed in 'Nishiuchi-Konatsu' and the common type. In self-pollinated 'Nishiuchi-Konatsu', the pollen tubes penetrated into upper part of the ovary. Hence, a few fertilized ovules were produced. In reciprocal pollinated 'Nishiuchi-Konatsu' and the common type and self-pollinated the common type, the pollen tubes penetrated only into middle part of the styles. Hence no fertilized ovules were produced. These data indicate that the seedlessness of 'Muroto-Konatsu' results from the inhibition of pollen tube growth into the pistil when cross-pollinated, whereas in 'Nishiuchi-Konatsu', which is partially self-compatible, it is nearly always seedless because of poor pollen growth when self-pollinated. We conclude that seedless cultivars 'Muroto-Konatsu' and 'Nishiuchi-Konatsu' mutated in the characters of the pistils on cross-compatible and self-incompatible, respectively.