Characterization of OsPID, the rice ortholog of PINOID, and its possible involvement in the control of polar auxin transport
Characterization of OsPID, the rice ortholog of PINOID, and its possible involvement in the control of polar auxin transport
复制标题
DOI：
10.1093/pcp/pcm024
复制
发表时间：
2007-03-01
影响因子：
4.9
通讯作者：
Kyozuka, Junko
中科院分区：
文献类型：
--
作者：
Morita, Yutaka;Kyozuka, Junko
PINOID 是拟南芥中的一种丝氨酸苏氨酸蛋白激酶，通过正控制 PIN 生长素流出载体的亚细胞定位来控制生长素分布。与对双子叶植物中生长素作用机制的快速了解相比，人们对单子叶植物中生长素作用知之甚少。在这里，我们描述了水稻的PINOID直系同源物OsPID的鉴定和表征。系统发育分析表明，水稻基因组包含一个 PID 直向同源基因 OsPID。 OsPID的组成型过度表达会引起多种异常，例如不定根发育延迟、芽的卷曲生长和向地性。在过度表达 OsPID 的植物中观察到的异常现象可以通过生长素活性极性转运抑制剂的处理进行表型复制，这表明 OsPID 可能参与了水稻中极性生长素转运的控制。 OsPID mRNA 分布分析显示，芽分生组织中存在复杂的模式，表明它可能在水稻芽的模式形成和器官发生中发挥作用。
PINOID, a serine threonine protein kinase in Arabidopsis, controls auxin distribution through a positive control of subcellular localization of PIN auxin efflux carriers. Compared with the rapid progress in understanding mechanisms of auxin action in dicot species, little is known about auxin action in monocot species. Here, we describe the identification and characterization of OsPID, the PINOID ortholog of rice. Phylogenetic analysis showed that the rice genome contains a single PID ortholog, OsPID. Constitutive overexpression of OsPID caused a variety of abnormalities, such as delay of adventitious root development, curled growth of shoots and agravitropism. Abnormalities observed in the plants that overexpress OsPID could be phenocopied by treatment with an inhibitor of active polar transport of auxin, indicating that OsPID could be involved in the control of polar auxin transport in rice. Analysis of OsPID mRNA distribution showed a complex pattern in shoot meristems, indicating that it probably plays a role in the pattern formation and organogenesis in the rice shoot.